Bio-Rad MCA1849T MARCO抗体,Rat anti mouse MARCO-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1849T MARCO抗体,Rat anti mouse MARCO

2024-10-22

Rat anti mouse MARCO

货号：MCA1849T ,MCA1849

规格：25 µg,0.25 mg

价格：663,2933

产品类型：流式抗体

品牌：Bio-Rad

抗原：MARCO

物种：小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：ED31

荧光染料：Purified

产品介绍
Rat anti Mouse MARCO antibody, clone ED31recognizes the murine cell surface antigen designated MARCO (Macrophage receptor with collagenous structure), which is a member of the class A scavenger receptor family.MARCO is expressed by distinct populations of macrophages in the spleen and lymph nodes, but is rapidly induced on macrophages in other tissues (e.g. Liver Kupffer cells) during infection or LPS treatment.Rat anti Mouse MARCO antibody, clone ED31 binds to the C-terminal cysteine rich domain of MARCO, and has been shown to block ligand binding.
产品详情
Target Species	Mouse
Product Form	PurifiedIgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Immunogen	CHO cells expressing mouse MARCO.
Approx. Protein Concentrations	IgG concentration 1 mg/ml
Fusion Partners	Spleen cells from immunised rats were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	x
Immunofluorescence	√
Immunohistology - Frozen	√	1/200	1/400
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
数据
Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MCA1849) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). High power. Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MCA1849), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image wiith nuclei counterstained blue using DAPI. high power
文献
1. Mattsson, J.et al.(2011) Complement activation and complement receptors on follicular dendritic cells are critical for the function of a targeted adjuvant.J Immunol. 187: 3641-52.2. Fukui Yet al.(2013) Effect ofLactobacillus brevisKB290 on the cell-mediated cytotoxic activity of mouse splenocytes: a DNA microarray analysis.Br J Nutr. 110 (9): 1617-29.3. Hayashi, M.et al.(2017) Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines.EBioMedicine. 15: 127-36.4. Martinez, N.et al.(2016) Impaired Recognition ofMycobacterium tuberculosisby Alveolar Macrophages From Diabetic Mice.J Infect Dis. 214 (11): 1629-1637.5. Parsa, R.et al.(2016) BAFF-secreting neutrophils drive plasma cell responses during emergency granulopoiesis.J Exp Med. 213 (8): 1537-53.6. Marrella, V.et al.(2015) IL-10 critically modulates B cell responsiveness in Rankl-/- mice.J Immunol. 194 (9): 4144-53.7. Kolan, S.S.et al.(2015) Lack of non-hematopoietic SIRPα signaling disturbs the splenic marginal zone architecture resulting in accumulation and displacement of marginal zone B cells.Biochem Biophys Res Commun. 460 (3): 645-50.8. Flores, M>et al.(2015) FcγRIIB prevents inflammatory type I IFN production from plasmacytoid dendritic cells during a viral memory response.J Immunol. 194 (9): 4240-50.9. Zhao, Z.et al.(2019) Neutrophil-Derived MRP14 Supports Plasma Cell Commitment and Protects Myeloma Cells from Apoptosis.J Immunol Res. 2019: 9561350.10. Hamasaki, M.et al.(2020) Transcriptional profiling of murine macrophages stimulated with cartilage fragments revealed a strategy for treatment of progressive osteoarthritis.Sci Rep. 10 (1): 7558.

技术参数

产品应用 IHC(F); IF ;WB

产品类型 Monoclonal Antibody

推荐用于 IHC(F); IF ;WB

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Bio-Rad MCA1849T MARCO抗体,Rat anti mouse MARCO

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