Bio-Rad MCA1821F CD79b抗体,Hamster anti mouse CD79b:FITC

2024-10-22

Hamster anti mouse CD79b:FITC

货号:MCA1821F

规格:0.1 mg

价格:2576

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD79b

物种:小鼠

宿主:Armenian hamster仓鼠

抗体亚型:IgG

克隆号:HM79-11

荧光染料:FITC

产品介绍
Hamster anti Mouse CD79b antibody, clone HM79-11recognizes murine CD79 beta, expressed by B cells as part of the B cell receptor complex (immunoglobulin and the CD79 alpha/beta heterodimer).Murine CD79 beta is expressed at the cell surface of pro-B cells prior to surface immunoglobulin, and is expressed throughout B cell differentiation.CD79 beta is a B cell specific marker, valuable for the detection of B cells at all maturation stages.
产品详情

Target Species

Mouse

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin

ImmunogenCD79 alpha/CD79 beta heterodimer purified from WEHI-231 B cells.
Fusion PartnersLymph node cells from immunised Armenian hamsters were fused with cells of the mouse X63-Ag8.653 myeloma cell line.
Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Max Ex/Em

FluorophoreExcitation Max (nm)Emission Max (nm)

FITC

490525

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
同型
DescriptionProduct CodeApplicationsPack Size

Hamster (Armenian) IgG Negative Control:FITC

MCA2356F

F

100 Tests

文献
1. Koyama, M.et al.(1997) CD79 alpha/CD79 beta heterodimers are expressed on pro-B cell surfaces without associated mu heavy chain.Int Immunol. 9 (11): 1767-72.2. Luger, D.et al.(2013) Expression of the B-cell receptor component CD79a on immature myeloid cells contributes to their tumor promoting effects.PLoS One. 8 (10): e76115.3. Jelínek, F.et al.(2005) Characteristics of two mouse bcr-abl-transformed cell lines. II. Pathological lesions induced in mice.Folia Biol (Praha). 51 (4): 93-102.4. Ankeny, D.P.et al.(2006) Spinal cord injury triggers systemic autoimmunity: evidence for chronic B lymphocyte activation and lupus-like autoantibody synthesis.J Neurochem. 99 (4): 1073-87.5. Lacoste-collin, L.et al.(2007) Effect of continuous irradiation with a very low dose of gamma rays on life span and the immune system in SJL mice prone to B-cell lymphoma.Radiat Res. 168 (6): 725-32.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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