Bio-Rad MCA1820 IL-4抗体,mouse anti bovine IL-4-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1820 IL-4抗体,mouse anti bovine IL-4

2024-10-22

mouse anti bovine IL-4

货号：MCA1820

规格：0.5 mg

价格：4012

产品类型：流式抗体

品牌：Bio-Rad

抗原：IL-4

物种：其它

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：CC303

荧光染料：Purified

产品介绍
Mouse anti Bovine Interleukin-4 antibody, clone CC303recognizes bovine interleukin 4
产品详情
Target Species	Bovine
Species Cross-Reactivity	Target Species	Cross Reactivity
Dog	√
Pig	√
Sheep	√
Mustelid	√
Goat	√
Dolphin	√
Mink	√
Fin Whale	√
Horse	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Approx. Protein Concentrations	IgG concentration 1.0mg/ml
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry1	√	1/50	1/100
ELISA	√
Western Blotting	X
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™(Product CodeBUF09) for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls. Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG2a Negative Control	MCA929	F	100 Tests
相关产品
Description	Product Code	Applications	Pack Size
Human Seroblock	BUF070A	F	50 Test
Human Seroblock	BUF070B	F	200 Test
文献
1. Costa-Pereira, C.et al.(2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.BMC Vet Res. 11 (1): 92.2. Dean, G.S.et al.(2005) Minimum infective dose ofMycobacterium bovisin cattle.Infect Immun. 73 (10): 6467-71.3. Araújo, M.S.et al.(2009) T-cell-derived cytokines, nitric oxide production by peripheral blood monocytes and seric anti-Leishmania (Leishmania) chagasi IgG subclass patterns following immunization against canine visceral leishmaniasis using Leishvaccine and Leishmune.Vaccine. 27 (7): 1008-17.4. Yang, J.et al.(2012) Comparison of worm development and host immune responses in natural hosts ofSchistosoma japonicum, yellow cattle and water buffalo.BMC Vet Res. 8: 25.5. Moreira, M.L.et al.(2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.Vet Parasitol. 220: 33-45.6. Geherin, S.A.et al.(2013) Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7.Vet Immunol Immunopathol. 155 (1-2): 87-97.7. Aguiar-Soares, R.D.O.et al.(2020) Phase I and II Clinical Trial Comparing the LBSap, Leishmune®, and Leish-Tec®Vaccines against Canine Visceral Leishmaniasis.Vaccines (Basel). 8 (4)Nov 17 [Epub ahead of print].8. Blanco, C.F.et al.(2021) Semi-stable production of bovine IL-4 and GM-CSF in the mammalian episomal expression systemJournal of Veterinary Research. 65, Aug 19 [Epub ahead of print]9. Villa-Mancera, A.et al.(2021) Phage display-based vaccine with cathepsin L and excretory-secretory products mimotopes ofFasciola hepatica.induces protective cellular and humoral immune responses in sheep.Vet Parasitol. 289: 109340.

技术参数

产品应用 ELISA;Flow

产品类型 Monoclonal Antibody

推荐用于 ELISA;Flow

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Bio-Rad MCA1820 IL-4抗体,mouse anti bovine IL-4

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