Bio-Rad MCA1730F CD44v6抗体,mouse anti human CD44v6:FITC

2024-10-22

mouse anti human CD44v6:FITC

货号:MCA1730F

规格:100 Tests

价格:3247

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD44v6

物种:人/恒河猴

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:VFF-7

荧光染料:FITC

产品介绍

Mouse anti Human CD44v6 antibody, clone VFF-7recognizes an epitope encoded by exon v6 on the variant portion of human CD44.
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Buffer SolutionTris buffered saline
Preservative Stabilisers

0.02%Sodium Azide

1%Bovine Serum Albumin

ImmunogenGlutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.
Approx. Protein ConcentrationsIgG concentration 0.1mg/ml
Fusion PartnersSpleen cells from immunized BALB/c mice were fused with cells of the P3X63Ag8.653 myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

应用

Application Name

Verified

Min Dilution

Max Dilution

Flow Cytometry

Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
数据

Immunoperoxidase staining of formalin fixed, paraffin embedded human breast cancer tissue with Mouse anti Human CD44v6 antibody (MCA1730). Low power.

同型

Description

Product Code

Applications

Pack Size

Mouse IgG1 Negative Control:FITC

MCA928F

Flow

100 Tests

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文献
1.Neumayer, R.et al.(1999) CD44 expression in benign and malignant colorectal polyps.Dis Colon Rectum. 42 (1): 50-5.2.Koopman, G.et al.(1993) Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.J Exp Med. 177 (4): 897-904.3.Yoo, S.H.et al.(2005) Expression patterns of markers for type II pneumocytes in pulmonary sclerosing hemangiomas and fetal lung tissues.Arch Pathol Lab Med. 129 (7): 915-9.4.Kim, J.S. and Werth, V.P. (2011) Identification of specific chondroitin sulfate species in cutaneous autoimmune disease.J Histochem Cytochem. 59: 780-90.5.Chaiyarit, P.et al.(2008) Alteration of the expression of CD44 isoforms in oral epithelia and saliva from patients with oral lichen planus.J Clin Immunol. 28: 26-34.6.Shirure, V.S.et al.(2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.Am J Physiol Cell Physiol. 308 (1): C68-78.7.Noori, M.S.et al.(2018) An adhesion based approach for the detection of esophageal cancer.Integr Biol (Camb). 10 (12): 747-57.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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