bio-rad MCA1746APC Mouse anti Pig CD31:APC,Mouse anti Pig CD31:APC

2024-10-22

Mouse anti Pig CD31:APC

货号:MCA1746APC

规格:100 Tests

价格:4278

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD31

物种:pig

宿主:小鼠

抗体亚型:IgG1

克隆号:LCI-4

荧光染料:APC

产品介绍
Mouse anti Pig CD31, clone LCI-4recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types (Fawcwettet al.1995). The extracellular region contains six Ig-like domains. Mouse anti Pig CD31, clone LCI-4 is cross reactive with human CD31 and binds to the 5thextracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutants (Nasuet al.1999).Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions (Nasuet al.1999).
产品详情
Target SpeciesPig
Species Cross-ReactivityTarget SpeciesCross Reactivity
Human
Mouse×
N.B. Antibody reactivity and working conditions may vary between species.
Product FormPurified IgG conjugated to Allophycocyanin (APC) - lyophilized
ReconstitutionReconstitute with 1ml distilled water
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
APC650661
ImmunogenPorcine CD31/human IgGFc fusion protein.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃.After reconstitution store at +4℃.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Nasu, K.et al.(1999) Alpha-galactosyl-mediated activation of porcine endothelial cells: studies on CD31 and VE-cadherin in adhesion and signaling.Transplantation. 68: 861-7.2.Evans, P.C.et al. (2001) Signaling through CD31 protects endothelial cells from apoptosis.Transplantation. 71 (3): 343-4.3.Gesslein, B.et al.(2010) Mitogen-activated protein kinases in the porcine retinal arteries and neuroretina following retinal ischemia-reperfusion.Mol Vis. 16: 392-407.4.Gyöngyösi, M.et al. (2010) Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion.Thromb Haemost. 104 (2): 376-84.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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