Bio-Rad MCA1723 CD8 beta抗体,mouse anti human CD8 beta-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1723 CD8 beta抗体,mouse anti human CD8 beta

2024-10-22

mouse anti human CD8 beta

货号：MCA1723

规格：0.2 mg

价格：4930

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD8 beta

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：2ST8.5H7

荧光染料：Purified

产品介绍
Mouse anti Human CD8 beta antibody, clone 2ST8.5H7recognizes the human CD8 cell surface antigen. The antibody binds to the beta chain, recognizing an epitope that is dependent upon expression of both CD8 alpha and CD8 beta.CD8 molecules are composed of dimers of either alpha/alpha or alpha/beta polypeptide combinations. Both types of dimer may co-exist at the cell surface. Clone 2ST8.5H7 is also referred to as clone T8/2T8-5H7 and binding is dependent on the expression of the alpha/beta dimer.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Cynomolgus monkey	√
Rhesus Monkey	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - lyophilized
Reconstitution	Reconstitute with 0.2ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide
Preparation	Purified IgG prepared by affinity chromatography from Ascites
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	1% Bovine Serum Albumin
Immunogen	Normal human T lymphocytes.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory	For research purposes only
Guarantee	Guaranteed until date of expiry. Please see product label.
存储条件
Prior to reconstitution store at +4oC.After reconstitution store at -20oC.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/10
Immunoprecipitation	√
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 106cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG2a Negative Control	MCA929	Flow	100 Tests
文献
1. Yoshino, N.et al.(2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110.2. Fry, T.J.et al.(2002) IL-7 therapy dramatically alters peripheral T-cell homeostasis in normal and SIV-infected nonhuman primates.Blood. 101: 2294-9.3. Terry, L.A.et al. (1990) Differential expression and regulation of the human CD8 alpha and CD8 beta chains.Tissue Antigens 35: 82-91.4. DiSanto, J.P.et al.(1991) Generation of anti-human CD8 beta-specific antibodies using transfectants expressing mixed-species CD8 heterodimers.J Immunol Methods. 141 (1): 123-31.5. de la Calle-Martin, O.et al.(2001) Familial CD8 deficiency due to a mutation in the CD8 alpha gene.J Clin Invest. 108: 117-23.6. Schmitz, J.E.et al(1998) Expression of the CD8alpha beta-heterodimer on CD8(+) T lymphocytes in peripheral blood lymphocytes of human immunodeficiency virus- and human immunodeficiency virus+ individuals.Blood. 92: 198-206.

技术参数

产品应用 Flow； IP； WB

产品类型 Monoclonal Antibody

推荐用于 Flow； IP； WB

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Bio-Rad MCA1723 CD8 beta抗体,mouse anti human CD8 beta

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