BIO-RAD MCA1615T Mouse anti Human CD54,Mouse anti Human CD54

2024-10-22

Mouse anti Human CD54

货号:MCA1615T

规格:0.1 mg

价格:935

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:CD54

物种:人

宿主:小鼠

抗体亚型:IgG1

荧光染料:其它

产品介绍
Mouse anti Human CD54 antibody, clone 15.2recognizes the human CD54 cell surface antigen also known as intracellular Adhesion Molecule -1 (ICAM-1) or Major group rhinovirus receptor.CD54 is expressed by many cells following activation by inflammatory mediators. It is a 505 amino acid with an additional 27 amino acid signal peptide ~90 kDa single pass type I transmembrane glycoprotein bearing 5 Ig-like C2-type domains.Mouse anti Human CD54 antibody, clone 15.2 blocks CD54 function (Berendtet al.1992). Mouse anti Human CD54 antibody, clone 15.2 binds to an epitope on the N-terminal Ig-like domain within a region designated the L43 loop (Chakravorty and Craig 2005).
产品详情
Target SpeciesHuman
Species Cross-ReactivityTarget SpeciesCross Reactivity
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product FormPurified IgG - liquid
Preparation
  • MCA1615, MCA1615T: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
  • MCA1615GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenHuman monocytes
Preservative Stabilisers0.09% Sodium Azide (NaN3)
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0-Ag14 myeloma cell line
Approx. Protein ConcentrationsIgG concentration 1 mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA1/501/100
Immunohistology - Frozen1/501/100
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117FF0.5mg
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117AE,WB0.5mg
文献
1.Dransfield, I.et al. (1992) Interaction of leukocyte integrins with ligand is necessary but not sufficient for function.J Cell Biol. 116:1527-35.2.Berendt, A.et al. (1992) The binding site on ICAM-1 for plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1- binding site.Cell. 68: 71-81.3.Urquhart, P.et al.(2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.J Pharmacol Exp Ther. 321 (2): 656-62.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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