Bio-Rad MCA1710SBV515 CD20抗体,mouse anti human CD20:StarBright Violet 515-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1710SBV515 CD20抗体,mouse anti human CD20:StarBright Violet 515

2024-10-22

mouse anti human CD20:StarBright Violet 515

货号：MCA1710SBV515

规格：100 Tests/0.5ml

价格：3596

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD20

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：2H7

荧光染料：SBV515

产品介绍
Mouse anti Human CD20 antibody, clone 2H7recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein.The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells.The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyaket al.2002).
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Rhesus Monkey	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG conjugated to StarBright Violet 515 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
StarBright Violet 515	401	516
Regulatory	For research purposes only
Guarantee	12 months from date of despatch

存储条件
Store at +4°C. DO NOT FREEZE.This product should be stored undiluted.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/20
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 5ul of the suggested working dilution to label 10⁶cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
数据
Figure A. PE conjugated Mouse anti Human CD19 (MCA1940PE). Figure B. PE conjugated Mouse anti Human CD19 (MCA1940PE) and SBV515 conjugated Mouse anti Human CD20 (MCA1710SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on theZE5 Cell Analyzer.
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文献
1. Greig, B.et al.(2014) Stabilization media increases recovery in paucicellular cerebrospinal fluid specimens submitted for flow cytometry testing.Cytometry B Clin Cytom. 86: 135-8.2. van den Akker, E.et al.(2010) The majority of the in vitro erythroid expansion potential resides in CD34(-) cells, outweighing the contribution of CD34(+) cells and significantly increasing the erythroblast yield from peripheral blood samples.Haematologica. 95: 1594-8.3. Jaramillo, M.C.et al.(2015) Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cells.Free Radic Biol Med. 83: 89-100.4. Cecchinato, V.et al.(2017) Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization.J Immunol. 198 (1): 184-195.5. Kohler, S.L.et al.(2016) Germinal Center T Follicular Helper Cells Are Highly Permissive to HIV-1 and Alter Their Phenotype during Virus Replication.J Immunol. 196 (6): 2711-22.6. Grobárová Vet al.(2016) Quambalarine B, a Secondary Metabolite fromQuambalaria cyanescenswith Potential Anticancer Properties.J Nat Prod. 79 (9): 2304-14.7. Popov, J.et al.(2017) Unique therapeutic properties and preparation methodology of multivalent rituximab-lipid nanoparticles.Eur J Pharm Biopharm. 117: 256-69.8. Sieg, M.et al.(2019) A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood.Viruses. 11 (2) Feb 09 [Epub ahead of print].

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Bio-Rad MCA1710SBV515 CD20抗体,mouse anti human CD20:StarBright Violet 515

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