Bio-Rad MCA1557SBV670 CD105抗体,mouse anti human CD10:StarBright Violet 670-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1557SBV670 CD105抗体,mouse anti human CD10:StarBright Violet 670

2024-10-22

mouse anti human CD10:StarBright Violet 670

货号：MCA1557SBV670

规格：100 Tests/0.5ml

价格：4106

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD105

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：SN6

荧光染料：StarBright Violet 670

产品介绍
Mouse anti Human CD105 antibody, clone SN6recognizes human endoglin, also known as CD105. CD105 is a glycoprotein homodimer of ~95 kDa subunits expressed by endothelial cells, activated monocytes and some leukemia cells.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Horse	√
Cynomolgus monkey	√
Rhesus Monkey	√
Primate	Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG conjugated to StarBright Violet 670 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin 0.1% Pluronic F680.1% PEG 3350
Immunogen	Partially purified cell membrane antigens from fresh leukemia cells
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
StarBright Violet 670	400	667
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 5ul of the suggested working dilution to label 106cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
相关产品
Description	Product Code	Applications	Pack Size
Human Seroblock	BUF070A	Flow	50 Tests
Human Seroblock	BUF070B	Flow	200 Tests
数据
Figure A. FITC conjugated Mouse anti Human CD31 (MCA1738F). Figure B. FITC conjugated Mouse anti Human CD31 (MCA1738F) and StarBright Violet 670 conjugated Mouse anti Human CD105 (MCA1557SBV670). All experiments performed on HUVECs gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
文献
1.Pierelli, L.et al.(2000) Modulation of bcl-2 and p27 in human primitive proliferating hematopoietic progenitors by autocrine TGF-beta1 is a cell cycle-independent effect and influences their hematopoietic potential.Blood 95: 3001-9.2.May, J.E.et al.(2018) Chemotherapy-induced genotoxic damage to bone marrow cells: long-term implications.Mutagenesis. 33 (3): 241-251.3.Hauser, P.V.et al.(2010) Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.Am J Pathol. 177: 2011-21.4.Jin, H.J.et al.(2010) GD2 expression is closely associated with neuronal differentiation of human umbilical cord blood-derived mesenchymal stem cells.Cell Mol Life Sci. 67 (11): 1845-58.5.Nagano, M.et al.(2007) Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood.Blood 110 (1): 151-60.6.Braun, J.et al.(2010) Evaluation of the osteogenic and chondrogenic differentiation capacities of equine adipose tissue-derived mesenchymal stem cells.Am J Vet Res. 71 (10): 1228-36.7.Diaz-Romero, J.et al.(2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.J Cell Physiol. 214: 75-83.8.Agha-Hosseini, F.et al.(2010)In vitroisolation of stem cells derived from human dental pulp.Clin Transplant. 24: E23-8.9.Arufe, M.C.et al.(2010) Chondrogenic potential of subpopulations of cells expressing mesenchymal stem cell markers derived from human synovial membranes.J Cell Biochem. 111: 834-45.10.Balmayor, E.R.et al.(2011) Synthesis and functionalization of superparamagnetic poly-ε-caprolactone microparticles for the selective isolation of subpopulations of human adipose-derived stem cells.J R Soc Interface 8: 896-908.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Bio-Rad MCA1557SBV670 CD105抗体,mouse anti human CD10:StarBright Violet 670

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