Bio-Rad MCA1556SBV670 CD10抗体,mouse anti human CD10:StarBright Violet 670-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1556SBV670 CD10抗体,mouse anti human CD10:StarBright Violet 670

2024-10-22

mouse anti human CD10:StarBright Violet 670

货号：MCA1556SBV670

规格：100 Tests/0.5ml

价格：4106

产品类型：流式抗体

品牌：Bio-Rad

抗原：SN5c

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：CD10

荧光染料：StarBright Violet 670

产品介绍
Mouse anti Human CD10 antibody, clone SN5c recognizes human neprilysin, also known as CD10, atriopeptidase, enkephalinase, neutral endopeptidase 24.11, skin fibroblast elastase or common acute lymphocytic leukemia antigen (CALLA). CD10 is a 749 aminoacid, ~100 kDa single pass type II transmembrane glycoprotein expressed by acute lymphoblastic leukaemia cells and by peripheral blood granulocytes.Defects in the MME gene encoding CD10 can lead to the development of the peripheral nervous system disorder, Charcot-Marie-Tooth disease 2T (CMY2T), an axonal form of Marie-Charcot-Tooth disease characterized by either dominantly inherited primary peripheral demyelinating neuropathies, designated CMT1, or primary peripheral axonal neuropathies showing axonal degeneration in the absence of any obvious myelin alteration (CMT2).
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to StarBright Violet 670 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 0.1% Pluronic F68 0.1% PEG 3350 0.05% Tween 20
Immunogen	Partially purified cell membrane antigens from fresh leukemia cells
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse PS/NS1/1-Ag4-1 myeloma cell line
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
StarBright Violet 670	400	667
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Store at +4°C. DO NOT FREEZE.This product should be stored undiluted.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 5ul of the suggested working dilution to label 106cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
相关产品
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Human Seroblock	BUF070A	Flow	50 Tests
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数据
Figure A. FITC conjugated Mouse anti Human CD11b (MCA551F). Figure B. FITC conjugated Mouse anti Human CD11b (MCA551F) and StarBright Violet 670 conjugated Mouse anti Human CD10 (MCA1556SBV670). All experiments performed on red cell lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
文献
1.Biddle, W.C.et al.(1989)In vitroandin vivocytotoxic activity of anti-human leukemia monoclonal antibodies SN5c and SN6 daunorubicin conjugates.Leuk Res. 13 (8): 699-707.2. Riemann, D.et al.(2001) Caveolae/lipid rafts in fibroblast-like synoviocytes: ectopeptidase-rich membrane microdomains.Biochem J. 354 (Pt 1): 47-55.3. Diaz-Romero, J.et al.(2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflectbona fidededifferentiation rather than amplification of progenitor cells.J Cell Physiol. 214: 75-83.4. Pilling, D.et al.(2009) Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.PLoS One. 4 (10): e7475.5. Manini, I.et al.(2020) Heterogeneity Matters: Different Regions of Glioblastoma Are Characterized by Distinctive Tumor-Supporting Pathways.Cancers (Basel). 12 (10)Oct 13 [Epub ahead of print].6. Glynn, E. & Fromm, J.R. (2020) Immunophenotypic Characterization and Purification of Neoplastic Cells from Lymph Nodes Involved by T-Cell/Histiocyte-rich Large B-cell Lymphoma by Flow Cytometry and Flow Cytometric Cell Sorting.Cytometry B Clin Cytom. 98 (1): 88-98.7. Caponnetto, F.et al.(2020) Human Adipose-Derived Stem Cells in Madelung's Disease: Morphological and Functional Characterization.Cells. 10 (1): 44.8. Kirolos, S.A. and Gomer, R.H. (2022) The extracellular sialidase NEU3 induces neutrophil primingbioRxiv. 24 Feb [Epub ahead of print].

技术参数

产品应用 Flow Cytometry

产品类型 Monoclonal Antibody

推荐用于 Flow Cytometry

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Bio-Rad MCA1556SBV670 CD10抗体,mouse anti human CD10:StarBright Violet 670

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