Bio-Rad MCA1429GA Lamin A/C 抗体,Mouse anti Human lamin A/C, clone JOL2-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1429GA Lamin A/C 抗体,Mouse anti Human lamin A/C, clone JOL2

2024-10-22

Mouse anti Human lamin A/C, clone JOL2

货号：MCA1429GA

规格：0.1 mg

价格：5398

产品类型：流式抗体

品牌：Bio-Rad

抗原：Lamin A/C

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号： JOL2

荧光染料：Purified

产品介绍
Mouse anti Human lamin A/C, clone JOL2recognizes both recombinant and native forms of human lamin A and C, nuclear intermediate filament proteins encoded by the same LMNA gene via alternative splicing. In addition to providing structural support to the nuclear envelope, these proteins contribute to chromatin organization and the regulation of gene expression.Mutations in the LMNA gene are responsible for a broad spectrum of disorders, termed laminopathies, including Emery-Dreifuss muscular dystrophy and dilated cardiomyopathy.Mouse anti Human lamin A/C, clone JOL2 has been shown to bind to an epitope between amino acids 464-572.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Xenopus
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN3)
Immunogen	Recombinant human lamin A
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2-0/Ag4 myeloma cell line
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
Immunofluorescence	√
Immunohistology - Frozen	√
Immunohistology - Paraffin 1	√		0.4 ug/ml
Immunoprecipitation	√
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. 1Mouse anti lamin A/C requires antigen retrieval using heat treatment prior to staining of paraffin sections. Citrate buffer pH 6.2 is recommended for this purpose. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Histology Positive Control Tissue Human colon
数据
Formalin fixed, paraffin embedded section of human colon stained with Mouse anti Lamin A/C (MCA1429GA).
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	F	0.1 mg
文献
1. Tilgner, K.et al.(2009) Dynamic complexes of A-type lamins and emerin influence adipogenic capacity of the cell via nucleocytoplasmic distribution of beta-catenin.J Cell Sci. 122: 401-13.2. Ivorra, C.et al.(2006) A mechanism of AP-1 suppression through interaction of c-Fos with lamin A/C.Genes Dev. 20: 307-20.3. Navarro, C.L.et al.(2004) Lamin A and ZMPSTE24 (FACE-1) defects cause nuclear disorganization and identify restrictive dermopathy as a lethal neonatal laminopathy.Hum Mol Genet. 13: 2493-503.4. Cummings, M.et al.(2006) XPA versus ERCC1 as chemosensitising agents to cisplatin and mitomycin C in prostate cancer cells: role of ERCC1 in homologous recombination repair.Biochem Pharmacol. 72: 166-75.5. Markiewicz, E.et al.(2006) The inner nuclear membrane protein emerin regulates beta-catenin activity by restricting its accumulation in the nucleus.EMBO J. 25: 3275-85.6. Zhang, Q.et al.(2007) Nesprin-1 and -2 are involved in the pathogenesis of Emery Dreifuss muscular dystrophy and are critical for nuclear envelope integrity.Hum Mol Genet. 16: 2816-33.7. Jean, D.et al.(2008) Cathepsin L expression is up-regulated by hypoxia in human melanoma cells: role of its 5'-untranslated region.Biochem J. 413: 125-34.8. Ndong, Jde. L.et al.(2009) Down-regulation of the expression of RB18A/MED1, a cofactor of transcription, triggers strong tumorigenic phenotype of human melanoma cells.Int J Cancer. 124: 2597-606.

技术参数

产品应用 IHC;IF;Flow；IP

产品类型 Monoclonal Antibody

推荐用于 IHC;IF;Flow；IP

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Bio-Rad MCA1429GA Lamin A/C 抗体,Mouse anti Human lamin A/C, clone JOL2

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