Bio-Rad MCA1427PE CD161抗体,mouse anti Rat CD161:PE-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1427PE CD161抗体,mouse anti Rat CD161:PE

2024-10-22

mouse anti Rat CD161:PE

货号：MCA1427PE

规格：100 Tests

价格：3783

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD161

物种：大鼠

宿主：小鼠

抗体亚型：IgG1

克隆号： 10/78

荧光染料：PE

产品介绍
Mouse anti Rat CD161 antibody, clone 10/78recognizes the rat Killer cell lectin-like receptor subfamily B protein, also known as NKR-PI or CD161. CD161 is a 233 amino acid ~60 kDa type II single pass protein containing a singleC-type lectindomain. CD161 is expressed on rat NK cells and T cell subpopulations. CD161 exists in 2 forms NKR-PIa and NKR-PIb, Mouse anti Rat CD161 antibody, clone 10/78 recognizes both forms of CD161 (Liet al.2003). Clone 10/78 competes with another anti CD161 clone, 3.2.3 for binding to antigen.Mouse anti Rat CD161 antibody, clone 10/78 has been successfully employed for thein vivodepletion of rat NK cells in an experimental obesity model (Wrannet al.2010).
产品详情
Target Species	Rat
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution	Reconstitute with 1 ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
Immunogen	Purified splenic NK cells from the LEW rat strain.
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.Ag8653 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 1 x10⁶cells in 100ul.
数据
Figure A. FITC conjugated mouse anti rat CD54 (MCA773F) and RPE conjugated mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated mouse anti rat CD54 (MCA773F) and RPE conjugated mouse anti rat CD161 (MCA1427PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:RPE	MCA1209PE	F	100 Tests
文献
1. Gondaira, S.et al.(2020) Immunosuppression in Cows following Intramammary Infusion ofMycoplasma bovis.Infect Immun. 88 (3) Feb 20 [Epub ahead of print].2. Khosa, S.et al.(2020) Bovine Adenovirus-3 Tropism for Bovine Leukocyte Sub-Populations.Viruses. 12 (12) Dec 12 [Epub ahead of print].3. Ramm, R.et al.(2020) Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovinein vivomodel.Xenotransplantation. 27 (2): e12571.4. Ramos, A.et al.(2018) Melatonin enhances responsiveness to Dichelobacter nodosus vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.Vet Immunol Immunopathol. 206: 1-8.5. Okino, C.H.et al.(2020) A polymorphic CD4 epitope related to increased susceptibility toBabesia bovis.in Canchim calves.Vet Immunol Immunopathol. 230: 110132.6. Park, D.S.et al.(2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.Cell Stress Chaperones. 26 (4): 705-20.7. Radley, G.et al.(2020)In Vitro.Benchmarking Study of Ventricular Assist Devices in Current Clinical Use.J Card Fail. 26 (1): 70-79.8. Colombatti Olivieri, M.A.et al.(2021) Evaluation of a virulent strain ofMycobacterium avium.subsp.paratuberculosis.used as a heat-killed vaccine.Vaccine. NNov 10;S0264-410X(21)01433-X.9. Casaro, S.et al.(2022) Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytesVet Immunol Immunopathol. 248: 110417.

技术参数

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Bio-Rad MCA1427PE CD161抗体,mouse anti Rat CD161:PE

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