Bio-Rad MCA1291P Kappa Light Chain抗体,Rat anti Mouse Kappa Light Chain:HRP

2024-10-22

Rat anti Mouse Kappa Light Chain:HRP

货号:MCA1291P

规格:0.5 mg

价格:7973

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Kappa Light Chain

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a

克隆号:LO-MK-1

荧光染料:HRP

产品介绍
Rat anti Mouse kappa light chain antibody, clone LO-MK-1recognises the murine kappa immunoglobulin light chain, and does not cross-react murine immunoglobulin heavy chains or lambda light chains.
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Preparation

Purified IgG prepared by affinity chromatography from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.01% Thiomersal

50% Glycerol

ImmunogenPurified mouse IgG1 kappa.
Approx. Protein ConcentrationsIgG concentration 1 mg/ml

Fusion Partners

Spleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line.

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution

ELISA

500ng/ml1000ng/ml
Immunohistology - Frozen
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • ELISA
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together withMCA1589as the capture reagent.
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文献
1. Delaunay, T.et al.(1990) Rat (and mouse) monoclonal antibodies. VIII. ELISA measurement of Ig production in mouse hybridoma culture supernatants.J Immunol Methods. 131 (1): 33-9.2. Savignac. M.et al.(2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.J Immunol. 185:7527-36.3. Laffont Set al.(2017) Androgen signaling negatively controls group 2 innate lymphoid cells.J Exp Med. pii: jem.20161807. [Epub ahead of print]

技术参数

产品应用 C ;E

产品类型 单克隆抗体

推荐用于 C ;E

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