Bio-Rad MCA1226APC CD45抗体,Mouse anti Human CD8:APC

2024-10-22

Mouse anti Human CD8:APC

货号:MCA1226APC,MCA1226APCT

规格:100 Tests,25 Tests/0.25ml

价格:4038,1896

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD8

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:LT8

荧光染料:APC

产品介绍
Mouse anti Human CD8 antibody, clone LT8recognizes the human CD8 cell surface glycoprotein expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells.The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
ReconstitutionPack Size: 100 Tests
Reconstitute with 1 ml distilled H20
Pack Size: 25 Tests/0.25mL

Reconstitute with 0.25 ml distilled water

Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Species Cross-Reactivity

Target SpeciesCross Reactivity
Marmoset
Chimpanzee
Cynomolgus monkey
Red-bellied Tamarin
N.B. Antibody reactivity and working conditions may vary between species.

Preparation

Purified IgG prepared by ion exchange chromatography

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

Pack Size: 100 Tests

0.09% Sodium Azide (NaN3)

1% Bovine Serum Albumin

Pack Size: 25 Tests/0.25ml

0.09% Sodium Azide (NaN3)

1% Bovine Serum Albumin

5% Sucrose

Immunogen

Normal human peripheral blood lymphocytes.

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.

Max Ex/Em

FluorophoreExcitation Max (nm)Emission Max (nm)

APC

650661

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 25 TestsPrior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells or cells or 100ul human whole blood.
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:APCMCA928APCF100 Tests/1ml
相关产品
DescriptionProduct CodeApplicationsPack Size
Human SeroblockBUF070AF50 Test
Human SeroblockBUF070BF200 Test
数据

Figure A. FITC conjugated mouse anti human CD3 (MCA463F) and APC conjugated Mouse IgG1 isotype control (MCA928APC). Figure B. FITC conjugated mouse anti human CD3 (MCA463F) and APC conjugated mouse anti human CD8 (MCA1226APC). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A)
文献
1. Nelson, M.et al.(2010) Characterization of lethal inhalational infection withFrancisella tularensisin the common marmoset (Callithrix jacchus).J Med Microbiol. 59: 1107-13.2. Gibbings, D.J.et al.(2007) CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses.BMC Immunol. 8: 12.3. Junker, A.et al.(2007) Multiple sclerosis: T-cell receptor expression in distinct brain regions.Brain. 130: 2789-99.4. Held, K.et al.(2011) Expression of herpes simplex virus 1-encoded microRNAs in human trigeminal ganglia and their relation to local T-cell infiltrates.J Virol. 85 (19): 9680-5.5. Hood SPet al.(2014) Changes in immune cell populations in the periphery and liver of GBV-B-infected and convalescent tamarins (Saguinus labiatus).Virus Res. 179: 93-101.6. Nelson, M. & Loveday, M. (2014) Exploring the innate immunological response of an alternative nonhuman primate model of infectious disease; the common marmoset.J Immunol Res. 2014: 913632.7. Manivannan, K.et al.(2016) CADM1/TSLC1 Identifies HTLV-1-Infected Cells and Determines Their Susceptibility to CTL-Mediated Lysis.PLoS Pathog. 12 (4): e1005560.8. Gross, C.C.et al.(2016) Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation.Proc Natl Acad Sci U S A. 113 (21): E2973-82.9. Dunham, J.et al.(2016) Blockade of CD127 Exerts a Dichotomous Clinical Effect in Marmoset Experimental Autoimmune Encephalomyelitis.J Neuroimmune Pharmacol. 11 (1): 73-83.10. Bughani, U.et al.(2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.PLoS One. 12 (7): e0180088.11. Philippens, I.H.et al.(2017) Acceleration of Amyloidosis byInflammation in the Amyloid-Beta Marmoset Monkey Model of Alzheimer's Disease.J Alzheimers Dis. 55 (1): 101-113.

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