Bio-Rad MCA1223PE CD8 Alpha抗体,mouse anti PIG wCD8 alpha:PE-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1223PE CD8 Alpha抗体,mouse anti PIG wCD8 alpha:PE

2024-10-22

mouse anti PIG wCD8 alpha:PE

货号：MCA1223PE

规格：100 Tests

价格：4378

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD8 Alpha

物种：pig

宿主：小鼠

抗体亚型：IgG2a

克隆号：MIL12

荧光染料：PE

产品介绍
Mouse anti Pig wCD8 alpha antibody, clone MIL12recognizes an epitope on the alpha chain of porcine wCD8. Clone MIl12 was clustered at the Third International Swine CD Workshop (Haversonet al.2001). Mouse anti Pig wCD8 alpha antibody, clone MIL12 was determined to bind to the CD8a epitope on the alpha chain based on its staining pattern on T lymphocytes and on its ability to block binding of the previously characterized CD8a antibody clone 76-2-11 to T lymphocytes (Saalmulleret al.2001).
产品详情
Target Species	Pig
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution	Reconstitute with 1.0 ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 5% Sucrose
Immunogen	Porcine Peripheral Blood lymphocytes.
Approx. Protein Concentrations	IgG concentration 0.1mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 106cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG2a Negative Control:RPE	MCA929PE	F	100 Tests/1ml
数据
Figure A. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti porcine CD5and RPE conjugated mouse anti porcine CD8a (MCA1223PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
文献
1. Stenfeldt, C.et al.(2014) Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A.Acta Vet Scand. 56: 42.2. Zeigler, B.M.et al.(2015) The development and validation of methods for evaluating the immune system in preweaning piglets.Food Chem Toxicol. 84: 197-207.3. Pasternak, J. A. (2014) Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal ModelJournal of Vaccines & Vaccination. 05 (05).4. Marinaro Met al.(2015) Changes in peripheral blood leucocytes of sheep experimentally infected withMycoplasma agalactiae.Vet Microbiol. 175 (2-4): 257-64.5. Liermann, W.et al.(2016) Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs.J Anim Physiol Anim Nutr (Berl). Nov 2. [Epub ahead of print]6. Hemmink, J.D.et al.(2016) Distinct immune responses and virus shedding in pigs following aerosol, intra-nasal and contact infection with pandemic swine influenza A virus, A(H1N1)09.Vet Res. 47 (1): 103.7. López, E.et al.(2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.BMC Vet Res. 15 (1): 91.8. Hu, Z.et al.(2019) Genomic variant in porcine TNFRSF1A gene and its effects on TNF signaling pathwayin vitro.Gene. 700: 105-9.9. Fogle, J.E.et al.(2019) Antibiotic Therapy Does Not Alter the Humoral Response to Vaccination for Porcine Circovirus 2 in Weaned Pigs.Vet Sci. 6 (2)May 30 [Epub ahead of print].10. Nielsen, O.L.et al.(2021) A porcine model of subcutaneousStaphylococcus aureus.infection: a pilot study.APMIS. Mar 01 [Epub ahead of print].

技术参数

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Bio-Rad MCA1223PE CD8 Alpha抗体,mouse anti PIG wCD8 alpha:PE

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