| 产品介绍 | | |
| Mouse anti Pig CD11R1, clone MIL4recognizes the porcine cell surface antigen classified as CD11R1 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haversonet al.2001). Mouse anti Pig CD11R1, clone MIL4 stains porcine eosinophils, a subset of neutrophils and NK cells, it does not stain monocytes or macrophages (Haversonet al.1994).Mouse anti Pig CD11R1, clone MIL4 immunoprecipitates a band corresponding to integrin β2 (CD18) of ~95kDa, in common with all other anti CD11 antibodies tested at the workshop and also a band of ~165 kDa corresponding to CD11R1, in a manner identical to the cross reactive anti human CD11b clone, TMG6-5 from peripheral blood mononuclear cell lysates suggesting that porcine CD11R1 is analogous to human CD11b (Dominguezet al.2001)Mouse anti pig CD11R1, clone MIL4 is cross reactive with the guinea pig and is useful for the identification of a population of guinea pig natural killer cells, Kurloff cells (Takizawaet al.2004) (Ereminet al.1980). |
| 产品详情 | | |
Target Species | Pig |
Product Form | Purified IgG - liquid |
Species Cross-Reactivity | Target Species | Cross Reactivity |
| Guinea Pig | √ |
| Human | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Carrier Free | Yes |
Immunogen | Porcine Lamina Propria Leucocytes. |
| Approx. Protein Concentrations | IgG concentration 1 mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/25 | 1/200 |
| Immunohistology - Frozen | √ | | |
| Immunoprecipitation | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 1x106cells in 100ul. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests/1ml |
| 数据 |
| Figure A. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse IgG1 isotype control (MCA928) detected with goat anti mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse anti porcine CD11R1 (MCA1220GA) detected with goat anti mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells. |
| 文献 |
| 1. Ordway, D.et al.(2007) The cellular immune response toMycobacterium tuberculosisinfection in the guinea pig.J Immunol. 179: 2532-41.2. Shang, S.et al.(2011) Activities of TMC207, rifampin, and pyrazinamide againstMycobacterium tuberculosisinfection in guinea pigs.Antimicrob Agents Chemother. 55 (1): 124-31.3. Rank, R.G.et al.(2012) Effect of Inflammatory Response on In Vivo Competition between Two Chlamydial Variants in the Guinea Pig Model of Inclusion Conjunctivitis.Infect Immun. 80: 612-9.4. Suda, Y.et al.(2014) ImmunobioticLactobacillus jenseniias immune-health promoting factor to improve growth performance and productivity in post-weaning pigs.BMC Immunol. 15: 24.5. Shegarfi, H.et al.(2015) Regulation of CCN1 (Cyr61) in a porcine model of intestinal ischemia/reperfusion.Innate Immun. 21 (5): 453-62.6. Auray Get al.(2016) Characterization and Transcriptomic Analysis of Porcine Blood Conventional and Plasmacytoid Dendritic Cells Reveals Striking Species-Specific Differences.J Immunol. Nov 11. pii: 1600672. [Epub ahead of print]7. Yeruva, L.et al.(2015) Chlamydial variants differ in ability to ascend the genital tract in the guinea pig model of chlamydial genital infection.Infect Immun. 83 (8): 3176-83.8. Sautter, C.A.et al.(2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.Dev Comp Immunol. 84: 181-92.9. Ferret-Bernard, S.et al.(2020) Maternal Supplementation of Food Ingredient (Prebiotic) or Food Contaminant (Mycotoxin) Influences Mucosal Immune System in Piglets.Nutrients. 12 (7): 2115.10. Iida, H.et al.(2019) Paraimmunobiotic Bifidobacteria Modulate the Expression Patterns of Peptidoglycan Recognition Proteins in Porcine Intestinal Epitheliocytes and Antigen Presenting Cells.Cells. 8(8):891. |
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