Bio-Rad AHP1833 CD120b,Rabbit anti Human CD120b

2024-10-22

Rabbit anti Human CD120b

货号:AHP1833

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:CD120b

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍

Rabbit anti human CD120brecognizes the soluble form of human CD120b, otherwise known as sTNF receptor type II (sTNFR1I), a member of the tumour necrosis factor receptor (TNFR) superfamily, produced by proteolytic cleavage of the membrane form by the metalloprotease TACE.CD120b plays a key role in regulating the inflammatory response, acting as a receptor for both tumour necrosis factor alpha (TNF-alpha) and TNF-beta, and is also involved in programmed cell death and anti-viral activity. sTNFR1I acts as an inhibitor of TNF-alpha induced responses, by acting as a decoy receptor.

产品详情

Target Species

Human

Product Form

Purified IgG - lyophilized

Antiserum Preparation

Antiserum to human CD120b were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

None present

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml after reconstitution

Immunogen

Recombinant human CD120b(PHP215)

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

Reconstitution

Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

0.5

2.0ug/ml

Functional Assays

15.020.0ug/ml
Immunofluorescence3.0ug/ml

Western Blotting

0.10.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • ELISA
  • This purified human CD120b antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with abiotinylated human CD120b antibody(AHP1833B) as the detection reagent andrecombinant human CD120b(PHP215) as the standard.
  • Western Blotting
  • This purified human CD120b antibody may be used in Western Blotting applications under either reducing or non-reducing conditions withrecombinant human CD120b(PHP215) as the positive control.
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Description

Product Code

Applications

Pack Size

Goat anti Rabbit IgG (Fc):Biotin

STAR121B

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Goat anti Rabbit IgG (Fc):FITC

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Flow

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Goat anti Rabbit IgG (Fc):HRP

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Goat anti Rabbit IgG (H/L):HRP

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Sheep anti Rabbit IgG:FITC

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数据

Immunofluorescent staining of CaCo-2 cells with Rabbit anti Human CD120b antibody (AHP1833), green. Counterstained with actin, red and nuclei, blue.

技术参数

产品应用 ELISA;FN;IF;WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;FN;IF;WB

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