Bio-Rad MCA1037F CD5抗体,Rat anti Dog CD5:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1037F CD5抗体,Rat anti Dog CD5:FITC

2024-10-22

Rat anti Dog CD5:FITC

货号：MCA1037F

规格：0.1 mg

价格：3723

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD5

物种：dog

宿主：大鼠

抗体亚型：IgG2a

克隆号：YKIX322.3

荧光染料：FITC

产品介绍
Rat anti Dog CD5 antibody, clone YKIX322.3recognizes canine CD5, a 67 kDa cell surface type 1 transmembrane glycoprotein also known as lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T-cells and thymocytes, CD5 is also expressed by NK cells at low levels (Huanget al.2008). Rat anti dog CD5, cloneYKIX322.3 was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop (Cobboldet al.1994).In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B-cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression (Vernau and Moore 1999). Rat anti dog CD5 serves as a useful marker for the discrimination of canine leukemias of differing origins (Deraviet al.2017).
产品详情
Target Species	Dog
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Immunogen	Concanavilin A activated canine peripheral blood cells
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
产品推荐
Description	Product Code	Applications	Pack Size
Rat IgG2a Negative Control:FITC	MCA1212F	F	100 Tests
文献
1.Cobbold, S/ & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.2.Hewicker-Trautwein, M.et al.(1999) Immunocytochemical demonstration of lymphocyte subsets and MHC class II antigen expression in synovial membranes from dogs with rheumatoid arthritis and degenerative joint disease.Vet Immunol Immunopathol. 67 (4): 341-57.3.Huang, Y.C.<i.et al.<="" i="" style="box-sizing: border-box;">(2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.J Leukoc Biol. 84: 1501-10.4.Araújo, M.S.et al.(2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.Vet Immunol Immunopathol. 141: 64-75.

技术参数

产品应用 Flow

产品类型单克隆抗体

推荐用于 Flow

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Bio-Rad MCA1037F CD5抗体,Rat anti Dog CD5:FITC

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