Rat anti Dog CD8 antibody, clone YCATE55.9 MCA1039A700 BIO-RAD ,Rat anti Dog CD8 antibody, clone YCATE55.9-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti Dog CD8 antibody, clone YCATE55.9 MCA1039A700 BIO-RAD ,Rat anti Dog CD8 antibody, clone YCATE55.9

2024-10-22

Rat anti Dog CD8 antibody, clone YCATE55.9

货号：MCA1039A700

规格：100 Tests/1ml

价格：4208

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD8

物种：dog

宿主：大鼠

抗体亚型：IgG1

克隆号：YCATE55.9

荧光染料：Alexa Fluor 700

产品介绍
Rat anti Dog CD8 antibody, clone YCATE55.9was clustered as Canine CD8 in the First Canine Leukocyte Antigen Workshop (Cobboldet al.1994). YCATE55.9 reacts with a rat cell line transfected with cDNA for canine CD8α (Gormanet al.1994) and blocks MHC class I dependant T-cell responsesin vitroandin vivo.Rat anti Dog CD8, clone YCATE55.9 has been shown to deplete circulating CD8+ T cells when administered to dogsin vivo. (Watsonet al.1993) Reduced levels of circulating CD8+ T cells has been associated with decreased survival times for dogs with osteosarcoma (Billeret al.2010).
产品详情
Target Species	Dog
Product Form	Purified IgG conjugated to Alexa Fluor® 700 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)
Immunogen	Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.
Approx. Protein Concentrations	IgG concentration 0.05 mg/ml
Fusion Partners	Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®700	702	723
Acknowledgements	This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/5
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
产品推荐
Description	Product Code	Applications	Pack Size
Rat IgG1 Negative Control:Alexa Fluor® 700	MCA6004A700	F	100 Tests/1ml
文献
1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.2.Gorman, S.D.et al.(1994) Isolation and expression of cDNA encoding the canine CD4 and CD8 alpha antigens.Tissue Antigens. 43 (3): 184-8.3.Watson, C.J.et al.(1993) CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog.Br J Surg. 80 (11): 1389-92.

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产品应用 flow

产品类型单克隆抗体

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Rat anti Dog CD8 antibody, clone YCATE55.9 MCA1039A700 BIO-RAD ,Rat anti Dog CD8 antibody, clone YCATE55.9

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