Mouse anti Mouse CD90 antibody, clone F7D5 BIO-RAD ,Mouse anti Mouse CD90 antibody, clone F7D5

2024-10-22

Mouse anti Mouse CD90 antibody, clone F7D5

货号:MCA02RT,MCA02R

规格:25 µg,0.25 mg

价格:808,3018

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD90

物种:小鼠

宿主:小鼠

抗体亚型:IgM

克隆号:F7D5

荧光染料:Purified

产品介绍
Mouse anti Mouse CD90 antibody, clone F7D5recognizes the mouse Thy1.2 alloantigen, also known as CD90.2, which is expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB.The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lakeet al.1979).Mouse anti Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes.
产品详情
Target SpeciesMouse
Product FormIgM fraction - liquid
PreparationIgM fraction prepared by ammonium sulphate precipitation
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)
Fusion PartnersSpleen cells from immunised AKR mice were fused with cells of the mouse NS-1 myeloma cell line.
Approx. Protein ConcentrationsIgM concentration 1.0 mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Cytotoxic Assays
Flow Cytometry1/101/25
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Human anti Mouse IgM:FITCHCA040FF0.1 mg
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138AE,WB1 ml
文献
1.Lake, P.et al.(1979) Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets.Eur J Immunol. 9 (11): 875-86.2.Yoshida, K.et al.(2002) Evidence for shared recognition of a peptide ligand by a diverse panel of non-obese diabetic mice-derived, islet-specific, diabetogenic T cell clones.Int Immunol. 14 (12): 1439-47.3.DeVries-vanDerZwan, A.et al.(1997) Specific tolerance induction and transplantation: a single-day protocol.Blood. 89 (7): 2596-601.4.Winzeler, A.M.et al.(2011) The lipid sulfatide is a novel myelin-associated inhibitor of CNS axon outgrowth.J Neurosci. 31: 6481-92.

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产品类型 单克隆抗体

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