Bio-Rad MCA1033A647 CD71抗体,Rat anti Mouse CD71:Alexa Fluor 647-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1033A647 CD71抗体,Rat anti Mouse CD71:Alexa Fluor 647

2024-10-22

Rat anti Mouse CD71:Alexa Fluor 647

货号：MCA1033A647,MCA1033A647T

规格：100 Tests/1ml,25 Tests/0.25ml

价格：3290,1233

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD71

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a

克隆号： YTA74.4

荧光染料：Alexa Fluor 647

产品介绍
Rat anti Mouse CD71 antibody, clone YTA74.4recognizes the mouse Transferrin receptor protein 1 also known as CD71 or TfR1. CD71 is a 763 amino acid glycoprotein homodimer of ~95 kDa subunits. CD71 is expressed by dividing cells, and functions as a transferrin receptor mediating uptake of iron.Rat anti Mouse CD71 antibody, clone YTA74.4 blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (Trowbridgeet al.1982).
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09%Sodium Azide1%Bovine Serum Albumin
Immunogen	Concanavilin A activated mouse spleen cells.
Approx. Protein Concentrations	IgG concentration 0.05 mg/ml
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®647	650	665
Fusion Partners	Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Acknowledgements	This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/5
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
文献
1.Millot, S.et al.(2010) Erythropoietin stimulates spleen BMP4-dependent stress erythropoiesis and partially corrects anemia in a mouse model of generalized inflammation.Blood. 116: 6072-81.2.Kuo, Y.M.et al.(2004) Mislocalisation of hephaestin, a multicopper ferroxidase involved in basolateral intestinal iron transport, in the sex linked anaemia mouse.Gut. 53: 201-6.3.Krysiak, K.et al.(2014) Reduced Levels of Hspa9 Attenuates Stat5 Activation in Mouse B-cells.Exp Hematol. pii: S0301-472X(14)00817-0.4.Byun, M.et al.(2007) Cowpox virus exploits the endoplasmic reticulum retention pathway to inhibit MHC class I transport to the cell surface.Cell Host Microbe. 2: 306-15.4.Ripich, T. and Jessberger, R. (2011) SWAP-70 regulates erythropoiesis by controlling α4 integrin.Haematologica. 96: 1743-52.

技术参数

产品应用 Flow

产品类型单克隆抗体

推荐用于 Flow

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Bio-Rad MCA1033A647 CD71抗体,Rat anti Mouse CD71:Alexa Fluor 647

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