LYNX Rapid RPE-Cy5 Antibody Conjugation Kit® LNK084C BIO-RAD ,LYNX Rapid RPE-Cy5 Antibody Conjugation Kit®-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

LYNX Rapid RPE-Cy5 Antibody Conjugation Kit® LNK084C BIO-RAD ,LYNX Rapid RPE-Cy5 Antibody Conjugation Kit®

2024-10-22

LYNX Rapid RPE-Cy5 Antibody Conjugation Kit®

货号：LNK084C,LNK082C

规格：3 Conjugations For 10µg Antibody,3 Conjugations For 60µg Antibody

价格：3247,6273

产品类型：抗体和染料

品牌：Bio-Rad

物种：人

宿主：其它

抗体亚型：其它

荧光染料：PE-Cy5

产品介绍
LYNX Rapid RPE-Cy5 Antibody Conjugation Kit®enables the rapid conjugation of a pre-prepared lyophilized mixture containing R-Phycoerythrin (RPE)-Cy5 label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in directional covalent bonding of RPE-Cy5 to the antibody.The LYNX Rapid Conjugation kit® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.
产品详情
Reagents in the Kit	Pack Size: 3 Conjugations For 60µg Antibody3 Vials of 100μg LYNX lyophilized RPE-Cy5 mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagentPack Size: 3 Conjugations For 10µg Antibody3 Vials of 10μg LYNX lyophilized RPE-Cy5 mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagent.
Preparing The Antibody	Pack Size: 3 Conjugations For 60µg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Due to the large size of the RPE-Cy5 label, it is recommended that 50-60ug of antibody be used for every 100μg RPE-Cy5, to ensure a slight RPE-Cy5 molar excess (50μg antibody gives a 1:1 Ab:RPE-Cy5 molar ratio). For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 60ul and a maximum antibody amount of 60ug. Antibody at a concentration of greater than 1mg/ml requires dilution. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit.Pack Size: 3 Conjugations For 10µg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Due to the large size of the RPE-Cy5 label, the quantity of RPE-tandem dye is in slight molar excess. Approximately 10ug of IgG will give a 1:1 molar ratio of antibody:RPE. For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 10ul and a maximum antibody amount of 10ug. Antibody at a concentration of greater than 1mg/ml requires dilution. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This kit contains lyophilized hygroscopic components that are moisture-sensitive. This kit is shipped under ambient conditions with silica packets to avoid exposure to moisture. On receipt, Bio-Rad recommend that the kit is stored at -20℃ and protected from moisture. Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing. Before opening, allow the components to reach room temperature to minimize condensation.
应用
Application Name	Verified	Min Dilution	Max Dilution
Conjugation	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. Instructions For Use 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25°C) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10ul of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.

技术参数

产品类型 Conjugation Kit

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LYNX Rapid RPE-Cy5 Antibody Conjugation Kit® LNK084C BIO-RAD ,LYNX Rapid RPE-Cy5 Antibody Conjugation Kit®

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