Human Anti-Human IgG1 Fc Antibody clone AbD27686 HCA285 bio-rad ,Human Anti-Human IgG1 Fc Antibody clone AbD27686-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Human Anti-Human IgG1 Fc Antibody clone AbD27686 HCA285 bio-rad ,Human Anti-Human IgG1 Fc Antibody clone AbD27686

2024-10-22

Human Anti-Human IgG1 Fc Antibody clone AbD27686

货号：HCA285

规格：0.1 mg

价格：2023

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：IgG1 Fc

物种：其它

宿主：人

抗体亚型：其它

克隆号：AbD27686

荧光染料：Purified

产品介绍
Human Anti-Human IgG1 Fc Antibody clone AbD27686is a recombinant monovalent Fab antibody, that recognizes human IgG1 Fc, with an affinity of 76 nM. It has been shown to have a greatly reduced affinity (89-fold) for IgG from cynomolgus macaque compared to human IgG1. It also has strongly reduced binding to rhesus macaque IgG. It does not recognize other human immunoglobulin isotypes (IgG2, IgG3, IgG4, IgA, IgE, IgM) or mouse IgG2a.This antibody has been generated by Bio-Rad using the HuCAL® antibody library and CysDisplay® technology. It is sold for research use, with implicit permission for use for the detection of therapeutic antibodies from samples obtained from experimental animals. When tested by Bio-Rad in an antigen capture pharmacokinetic assay format, this antibody performs similarly to Mouse Anti-Human IgG (CH2 Domain) Antibody clone R10Z8E9 (MCA5748G) in 10% cynomolgus macaque serum. It recognizes a different epitope on human IgG1 Fc than clone R10Z8E9.
产品详情
Target Species	Human
Product Form	A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL PLATINUM®phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.01% Thiomersal
Immunogen	Recombinant human IgG1 kappa
Approx. Protein ConcePurityntrations	IgG concentration 0.1 mg/ml
Affinity	The monovalent intrinsic affinity of this antibody was measured as KD=76 nM by real time, label free molecular interaction analysis on immobilized hIgG1 kappa
Acknowledgements	This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.His-tag is a registered trademark of EMD Biosciences.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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技术参数

产品应用 elisa

产品类型单克隆抗体

推荐用于 elisa

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Human Anti-Human IgG1 Fc Antibody clone AbD27686 HCA285 bio-rad ,Human Anti-Human IgG1 Fc Antibody clone AbD27686

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