Human anti Human DJ-1 antibody, clone AbD03055 HCA024 bio-rad,Human anti Human DJ-1 antibody, clone AbD03055

2024-10-22

Human anti Human DJ-1 antibody, clone AbD03055

货号:HCA024

规格:0.1 mg

价格:2023

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:DJ-1

物种:人

宿主:人

抗体亚型:IgG1 Lamda

克隆号:AbD03055

荧光染料:Purified

产品介绍
Human anti Human DJ-1 antibody, clone AbD03055recognizes the human DJ-1 oncogene only when it is oxidized on cysteine C106. DJ-1 plays roles in transcriptional regulation and reaction to anti-oxidative stress. The oxidation status of DJ-1, particularly at C106, modulates the function of DJ-1 (Canet-Aviléset al.2004). De-regulation of DJ-1 oxidation appears to be connected to the onset of conditions such as Parkinson's Disease (Abou-Sleimanet al.2003).
产品详情
Target SpeciesHuman
Product FormA bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library. Expressed inE. coliand purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
PreparationMetal chelate affinity chromatography
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.01% Thiomersal
ImmunogenDJ-1 peptide oxidized at C106 (sequence LIAAIC(SO3)AGPTA).
Approx. Protein ConcePurityntrationsAntibody concentration 0.5 mg/ml.
AcknowledgementsThis product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.By purchasing this product, you agree that your Name and Affiliation will be sent to Dr. Ariga, Hokkaido University, who first published this antibody in the journal Neuroscience Letters (Vol. 404, pp.166-169, 2006).His-tag is a registered trademark of EMD Biosciences.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA
Western Blotting
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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技术参数

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产品类型 单克隆抗体

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