Human anti Ki67 antibody, clone AbD02531 HCA006 bio-rad ,Human anti Ki67 antibody, clone AbD02531

2024-10-22

Human anti Ki67 antibody, clone AbD02531

货号:HCA006

规格:0.1 mg

价格:1913

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Ki67

物种:人

宿主:人

抗体亚型:HuCAL Fab bivalent

克隆号:AbD02531

荧光染料:Purified

产品介绍
Human anti Ki67 antibody, clone AbD02531,recognizes the sequence GFKELFQ, which can be found eight times on the human Ki67 protein: Amino acid positions 1104-1110, 1226-1232, 1348-1354, 1590-1596, 1956-1962, 2078-2084, 2318-2324, and 2439-2445.Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells.The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis revealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quiescent cells. Immunoblot analysis showed expression of 320- and 359-kDa proteins. Sequence analysis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular proliferation in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation. Clone AbD02531 is also known as clone 2531.
产品详情
Target SpeciesHuman
Product FormBivalent human recombinant Fab selected from the HuCAL®GOLD phage display library. Expressed inE. coliand purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain -- Liquid.
PreparationMetal chelate affinity chromatography
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.01% Thiomersal
ImmunogenPeptide derived from the human Ki-67 protein. Sequence GFKELFQTPG, coupled via a C-terminal cysteine to carrier proteins.
ActivityActivity was tested by indirect ELISA: The Ki-67 antigen peptide was coupled to human transferrin carrier protein. The coupled antigen (5 μg/ml) plus unrelated control proteins were immobilized on a microtiter plate. Specific binding was monitored by first adding HCA006 (2 μg/ml), then adding a secondary antibody (goat anti-human F(ab')2 fragment specific, AP conjugate. 1:5000 diluted). A fluorescent signal was created by adding the AP sub-strate Attophos. The signal on the antigen is at least 5-fold above background, whereas the signal on the control antigens is less than 1.5-fold above background.
AffinitykD = 2.2 nMThe monovalent intrinsic affinity of HCA004 was measured by BIAcore on the immobilized BMPR-1A extracellular domain.
Approx. Protein ConcePurityntrationsAntibody concentration 0.5 mg/ml.
AcknowledgementsThis product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.His-tag is a registered trademark of EMD Biosciences.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA2ug/ml
Immunofluorescence
Western Blotting5ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Human IgG F(ab')2:HRP0500-0099E,WB0.5 ml
Goat anti Human IgG F(ab')2:Alk. Phos.0500-0100E,WB0.5 ml
文献
1.Jarutat, T.et al.(2006) Isolation and comparative characterization of Ki-67 equivalent antibodies from the HuCAL phage display library.Biol. Chem. 387: 995-1003.2.Liu, S.K.et al.(2011) Delta-like ligand 4-notch blockade and tumor radiation response.J Natl Cancer Inst. 103 (23): 1778-98.3.Zarobkiewicz, M.K.et al.(2018) Decrease in Lipid Droplets in Adrenal Cortex of Male Wistar Rats after Chronic Exposure to Energy Drinks.Medicina (Kaunas). 54 (5):90.

技术参数

产品应用 elisa,if,wb

产品类型 单克隆抗体

推荐用于 Ki67

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