Quantum™ R-PE MESF Medium Level FCSC827A BIO-RAD ,Quantum™ R-PE MESF Medium Level

2024-10-22

Quantum™ R-PE MESF Medium Level

货号:FCSC827A,FCSC827B

规格:20 Tests,100 Tests

价格:7421,15241

产品类型:流式微球

品牌:Bio-Rad

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:PE

产品介绍
Quantum™ R-PE MESF Medium Levelis comprised of four calibrated fluorescent populations, with different levels of RPE fluorescence intensity, and one blank population of uniform microspheres that are approximately the size of human lymphocytes (7-9μm). The excitation and emission spectra of the microspheres match those of cell samples labeled with RPE.The levels of fluorescence intensity in the different kits (Low, Medium and High) are intended to span the intensity range of common cellular analyses. Medium level kits are used for many types of analyses, and nicely span the range of typical cell samples. Common analyses include those for many surface markers, including CD4 / CD8.
产品详情
Intended UseFCSC827A is used in the quantitation of RPE fluorescence intensity in Molecules of Equivalent Soluble Fluorochrome (MESF) units. When used in conjunction with Simply Cellular® microspheres this kit also allows quantitation of Antibody Binding Capacity (ABC).This kit enables fluorescence intensity of a sample to be directly quantified in terms of MESF units and for samples from different instruments to be accurately compared. The fluorescence intensity of each of the five populations in the kit have been calibrated against solutions of laser grade fluorescent dye in units of MESF RPE per microsphere. The Certified Blank™population is used to measure the fluorescence detection threshold of the instrument. Correct use of the kit enables:1) quantitation of fluorescence intensity of samples in terms of MESF;2) determination of instrument fluorescence detection threshold;3) determination of instrument linearity;4) data comparison over time and between multiple instruments.
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)0.01% Gelatin0.01% Tween 80
Reagents in the KitPack Size: 20 Tests1 x 1ml bottle of unlabeled microbeads4 x 1ml bottles of labeled microbeadsPack Size: 100 Tests1 x 5ml bottle of unlabeled microbeads4 x 5ml bottles of labeled microbeads
AcknowledgementsQuantum™ and Simply Cellular® are trademarks of Bangs Laboratories, INC.
RegulatoryFor research purposes only
GuaranteeGuaranteed until date of expiry. Please see product label.
存储条件
Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 FLOW

产品类型 Accessory Reagent

推荐用于 FLOW

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