TMB Signal+ BIO-RAD BUF054A,TMB Signal+

2024-10-22

TMB Signal+

货号:BUF054A,BUF054B

规格:100 ml,500 ml

价格:1530,4233

产品类型:辅助试剂

品牌:Bio-Rad

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:其它

产品详情
Intended UseBUF054A is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA detection systems as a substrate for horseradish peroxidase (HRP).BUF054A contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. TMB SIGNAL+ has been optimised to enable increased sensitivity whilst maintaining increased stability. BUF054A provides minimal background and rapid development.BUF054A produces a deep blue colour during the enzymatic degradation of H2O2by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue). However, for endpoint assays the reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
Product FormReady to use TMB solution - liquid
RegulatoryFor research purposes only
GuaranteeGuaranteed until date of expiry. Please see product label.
存储条件
Store at +4℃. DO NOT FREEZE.This product is photosensitive and should be protected from light.Avoid exposure to heat and contamination with metal ions or peroxidase.Store in bottles made of High Density Polyethylene (HDPE).
应用
Application NameVerifiedMin DilutionMax Dilution
ELISANeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 ELISA

产品类型 Accessory Reagent

推荐用于 ELISA

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