Rabbit anti Human Insulin-Like Growth Factor II antibody bio-rad,Rabbit anti Human Insulin-Like Growth Factor II antibody

2024-10-22

Rabbit anti Human Insulin-Like Growth Factor II antibody

货号:AHP776

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Insulin-Like Growth Factor II

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human Insulin-Like Growth Factor II antibodyrecognizes human IGF-II (Insulin-like Growth Factor II), a 67 amino acid mitogenic polypeptide and member of the Insulin gene family, synthesized by various foetal tissues during prenatal development, under the influence of placental lactogen.IGF-II is a growth factor which exhibits both paracrine and autocrine properties and signals by binding to the type 1 and type 2 insulin-like growth factor receptors (type 1 IGFR and type 2 IGFR), and also to the insulin receptor (IR). The availability of IGF-II is dependent upon its attachment to, and release from, various IGF binding proteins (IGFBPs), a relationship which has been investigated in breast cancer research (Singeret al.2004).
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to Biotin - lyophilized
ImmunogenRecombinant human IGF-II.
ReconstitutionReconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml after reconstitution
Antiserum PreparationAntisera to human IGF-II were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Preservative StabilisersNone present.
RegulatoryFor research purposes only
Guarantee6 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.52.0ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg
文献
1.Terasawa, H.et al.(1994) Solution structure of human insulin-like growth factor II; recognition sites for receptors and binding proteins.EMBO J. 13 (23): 5590-7.2.Singer, C.F.et al.(2004) Insulin-like growth factor (IGF)-I and IGF-II serum concentrations in patients with benign and malignant breast lesions: free IGF-II is correlated with breast cancer size.Clin Cancer Res. 10 (12 Pt 1): 4003-9.

技术参数

产品应用 wb;elisa

产品类型 Polyclonal Antibody

推荐用于 wb;elisa

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