Bio-Rad Caspase-9 p10抗体 AHP2419,Rabbit anti Caspase-9 p10

2024-10-22

Rabbit anti Caspase-9 p10

货号:AHP2419

规格:0.1 mg

价格:3213

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Caspase-9 p10

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍

Rabbit anti caspase-9 p10 antibodyrecognizes caspase-9, a member of the cysteine-aspartic acid protease family. The active form of caspase-9 is generated by cleavage of the pro-caspase-9 protein into fragments, known as the large and small fragments. The trigger for caspase-9 cleavage is the formation of the apoptosome (comprising of cytochrome c, APAF-1, dATP and pro-caspase-9), upon stimulation of the intrinsic apoptosis pathway.The rabbit anti caspase-9 antibody was raised against an internal peptide that is present in one of the small fragments (p10) generated in the pro-caspase-9 cleavage process at Asp330 (Zouet al.2002). The antibody will therefore detect pro-caspase-9 (as the motif is present) and the p10 caspase-9 fragment. The antibody can therefore be used to distinguish the p10 caspase-9 fragment from other pro-caspase-9 cleavage products.

产品详情

Target Species

Human

Species Cross-Reactivity

Target SpeciesCross Reactivity

Mouse √

Rat √

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography

AntiserumPreparation

Antiserum to caspase-9 p10 was raised by repeated immunisation of rabbits with highly purified antigen

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.1% Sodium Azide (NaN3)0.2% Gelatin

Immunogen

Peptide mapping to an internal region of human caspase-9 p10 subunit

Approx. Protein Concentrations

IgG concentration 0.2 mg/ml

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

应用

Application Name

Verified

Min Dilution

Max Dilution

Western Blotting

1/200

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Western Blotting
  • Rabbit anti caspase-9 p10 recognizes a band of approximately 47 kDa when run under reducing conditions without induction of apoptosis

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