Bio-Rad WNT-3a抗体 AHP2188B,Rabbit anti Human WNT-3a:Biotin

2024-10-22

Rabbit anti Human WNT-3a:Biotin

货号:AHP2188B

规格:50 µg

价格:4208

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:WNT-3a

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Biotin

产品介绍

Rabbit anti Human RIP2 antibodyrecognizes Human Receptor-interacting serine/threonine-protein kinase 2, also known as RIP2, RIPK2, RIP-like-interacting CLARP kinase or CARD-containing interleukin-1 beta-converting enzyme-associated kinase. RIP2 is a 403 amino acid ~60 kDa cytoplasmic protein belonging to the protein kinase superfamily.RIP2 is a kinase that regulates TNF and Fas induced-apoptosis and is involved in the generation of the pro-inflammatory cytokine IL-1beta.Over expression of RIP2 has been shown to induce apoptosis, and the activation of NF-Kappa-B and JNK. RIP2 is widely expressed in a range of human tissues.

产品详情

Target Species

Human

Product Form

Purified IgG conjugated to Biotin - lyophilized

Reconstitution

Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albuminCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

AntiserumPreparation

Antiserum to human WNT-3a were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

None present

Immunogen

Recombinant human WNT-3a.

Approx. Protein Concentrations

IgG concentration 0.1mg/ml after reconstitution

Regulatory

For research purposes only

Guarantee

Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

存储条件

Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

0.25

1.0ug/ml

Western Blotting

0.1

0.2ug/ml

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • ELISA
  • AHP2188B may be used in a direct ELISA or as the detection antibody in a sandwich ELISA with (AHP2188) as the capture reagent.

文献

1.Lai, Y.H.et al.(2011) SOX4 interacts with plakoglobin in a Wnt3a-dependent manner in prostate cancer cells.BMC Cell Biol. 12: 50.2.Yan, L.et al.(2011) Activation of the canonical Wnt/β-catenin pathway in ATF3-induced mammary tumors.PLoS One. 6:e16515.

技术参数

产品应用 E; WB

产品类型 Polyclonal Antibody

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