Bio-Rad AHP1879 tyrosine hydroxylase,Sheep anti Rat tyrosine hydroxylase antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP1879 tyrosine hydroxylase,Sheep anti Rat tyrosine hydroxylase antibody

2024-10-22

Sheep anti Rat tyrosine hydroxylase antibody

货号：AHP1879

规格：0.1 ml

价格：7123

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：tyrosine hydroxylase

物种：大鼠

宿主：Sheep

抗体亚型：IgG

荧光染料：Purified

产品介绍
Sheep anti Rat tyrosine hydroxylase antibodyrecognizes rat tyrosine 3-monooxygenase, also known as tyrosine hydroxylase or tyrosine 3-hydroxylase. Tyrosine hydroxylase is a 497 amino acid ~56 kDa enzyme involved in the production of dopamine from L-tyrosine. Tyrosine hydroxylase also catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons.
产品详情
Target Species	Rat
Species Cross-Reactivity	Target Species	Cross Reactivity
Mammals	Expected from Sequence
Rabbit	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Antiserum Preparation	Antisera to tyrosine hydroxylase were raised by repeated immunisations of sheep with highly purified antigen.Purified IgG prepared by immunoaffinity chromatography.
Buffer Solution	10mM HEPES pH7.5, 150mM NaCl
Preservative Stabilisers	0.09% Sodium Azide (NaN3)0.01% Bovine Serum Albumin50% Glycerol
Immunogen	Rat Tyrosine hydroxylase purified from pheochromocytoma.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence	√		1/1000
Immunohistology - Frozen	√		1/1000
Western Blotting	√		1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Western BlottingAHP1879 detects a band of approximately 60kDa in rat caudate cell lysates.
数据
Western blot of rat caudate lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein using Sheep anti Rat tyrosine hydroxylase antibody (AHP1879).
文献
1. Xu, Z.Q.et al.(1998) Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies.Neuroscience. 82 (3): 727-38.2. Olivas, A.et al.(2016) Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130.J Neurosci. 36 (2): 479-88.

技术参数

产品应用 C;IF;WB

产品类型 Polyclonal Antibody

推荐用于 C;IF;WB

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Bio-Rad AHP1879 tyrosine hydroxylase,Sheep anti Rat tyrosine hydroxylase antibody

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