Rabbit anti Human interleukin-17F antibody AHP1283 bio-rad,Rabbit anti Human interleukin-17F antibody

2024-10-22

Rabbit anti Human interleukin-17F antibody

货号:AHP1283

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:interleukin-17F

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human interleukin-17F antibodyrecognizes human IL-17F (interleukin-17F), a disulphide-linked homodimer consisting of two 133 amino acid chains, which belongs to the IL-17 gene family and shares the highest sequence homology with IL-17A.IL-17F is expressed by activated CD4+ T-cells and monocytes, and acts as a stimulator of cytokine production, including granulocyte colony-stimulating factor (GM-CSF), IL-6 and IL-8. IL-7F also stimulates the production of IL-2, MCP-1 (monocyte chemotactic protein-1) and TGFB (Transforming growth factor beta) by endothelial cells, and inhibits endothelial cell angiogenesis.Rabbit anti Human interleukin-17F antibody (AHP1283) has been used in neutralisation assays where 50% inhibition (ND50) of the biological activity of 50ng/ml of human IL-17F was achieved using 0.9-1.2μg/ml of antibody.
产品详情
Target SpeciesHuman
Product FormPurified IgG - lyophilized
ReconstitutionReconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide.
Antiserum PreparationAntisera to human IL-17F were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer SolutionPhosphate buffered saline
Preservative StabilisersNone present
ImmunogenRecombinant human IL-17F.
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.52.0ug/ml
Functional Assays0.91.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg
文献
1.Starnes, T.et al.(2001) Cutting edge: IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production.J Immunol. 167 (8): 4137-40.

技术参数

产品应用 elisa;wb

产品类型 Polyclonal Antibody

推荐用于 elisa;wb

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