Goat anti Human Apolipoprotein B antibody AHP1267 bio-rad,Goat anti Human Apolipoprotein B antibody

2024-10-22

Goat anti Human Apolipoprotein B antibody

货号:AHP1267

规格:0.1 mg

价格:2831

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Apolipoprotein B

物种:人

宿主:山羊

抗体亚型:IgG

荧光染料:Purified

产品介绍
Goat anti Human Apolipoprotein B antibodydetects human Apolipoprotein B (ApoB), a large secretory protein with a molecular weight of ~550 kDa that plays a central role in lipoprotein metabolism.ApoB exists in the plasma in 2 main isoforms, ApoB100 and ApoB48. ApoB100 is synthesized in the liver, and is the major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL), and is absolutely required for their formation. ApoB100 acts as a ligand for LDL receptors in various cells throughout the body, allowing cells to internalize LDL and absorb cholesterol.ApoB48 is expressed in the intestine and is present on chylomicrons and their remnants, and lacks the C-terminal LDL-receptor binding region of ApoB100. Elevated plasma concentrations of ApoB containing lipoproteins are key risk factors for the development of atherosclerosis and myocardial infarction (Marstonet al. 2021).
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to Biotin - lyophilized
Antiserum PreparationAntisera to Apolipoprotein B were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution125mM Sodium Borate75mM Sodium Chloride
Preservative Stabilisers<0.1% Sodium Azide (NaN3)5mM EDTA
ImmunogenApolipoprotein type B, isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA1/20001/10,000
Immunoprecipitation
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Rabbit anti Goat IgG (Fc):FITCSTAR122FF100 Tests
Rabbit anti Goat IgG (Fc):HRPSTAR122PC,E,WB100 Tests
文献
1.Philippova, M.et al.(2019) Analysis of fragmented oxidized phosphatidylcholines in human plasma using mass spectrometry: Comparison with immune assays.Free Radic Biol Med. 144: 167-75.

技术参数

产品应用 elisa;wb

产品类型 Polyclonal Antibody

推荐用于 elisa;wb

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