Bio-Rad AHP1429 STIM2,Rabbit anti STIM2 antibody

2024-10-22

Rabbit anti STIM2 antibody

货号:AHP1429

规格:0.1 mg

价格:4616

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:STIM2

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti STIM2 antibodyrecognizes an internal region of stromal interaction molecule-2 (STIM2), a ubiquitously expressed type I membrane protein, of which at least three isoforms have been identified.In T-lymphocytes and other haematopoietic cells, the mechanism for increasing intracellular Ca2+, following antigen mediated T-cell activation, is through the store-operated Ca2+release-activated Ca2+(CRAC) channels. STIM1 is reported to activate CRAC channels upon Ca2+depletion. Whereas, STIM2 acts to inhibit the STIM1-mediated store-operated Ca2+entry, and forms complexes with STIM1, suggesting that these molecules may play a coordinated role in controlling Ca2+entry.
产品详情

Target Species

Human

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human STIM2 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02%Sodium Azide

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Immunogen

A 17 amino acid peptide located near the centre of human STIM2.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用

Application Name

Verified

Min Dilution

Max Dilution

Immunohistology - Paraffin1

Western Blotting

0.51.0ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Western Blotting
  • AHP1429 detects a band of approximately 105kDa in A20 cell lysates
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Description

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数据

Formalin fixed, paraffin embedded human spleen stained with Rabbit anti Human STIM2 (AHP1429) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0).

技术参数

产品应用 IHC(P)*;WB

产品类型 Polyclonal Antibody

推荐用于 IHC(P)*;WB

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