Bio-Rad AHP1156 DKK1,Rabbit anti Human DKK1 antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP1156 DKK1,Rabbit anti Human DKK1 antibody

2024-10-22

Rabbit anti Human DKK1 antibody

货号：AHP1156

规格：50 µg

价格：6503

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：DKK1

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Purified

产品介绍
Rabbit anti Human DKK1 antibodyrecognises human Dickkopf (Xenopus laevis) homolog 1 (DKK1), a 35 kDa to 50 kDa member of the dickkopf family. It is expressed in the placenta, prostate and to a lesser extent, in the colon and spleen. DKK1 is secreted when expressed in 293T cells.DKK1 inhibits Wnt signalling in embryonic development, most likely acting upstream of frizzled. It is also thought to contribute to neoplastic processes, inhibit osteoblast differentiation and is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.1% Sodium Azide (NaN₃)
Approx. Protein Concentrations	IgG concentration 1.0mg/ml
Immunogen	Synthetic human DKK1 - KLH conjugated.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin1	√		10ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Histology Positive Control Tissue Uterus, endometrium.
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数据
Staining of formalin fixed, paraffin embedded human endometrium with Rabbit anti Human DKK1 (AHP1156).
文献
1. Krupnik, V.E.et al.(1999) Functional and structural diversity of the human Dickkopf gene family.Gene. 238 (2): 301-13.2. Fedi, P.et al.(1999) Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling.J Biol Chem. 274 (27): 19465-72.3. Niehrs C. (2006) Function and biological roles of the Dickkopf family of Wnt modulators.Oncogene. 25:7469-81.

技术参数

产品应用 IHC(P)*

产品类型 Polyclonal Antibody

推荐用于 IHC(P)*

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Bio-Rad AHP1156 DKK1,Rabbit anti Human DKK1 antibody

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