Rabbit anti Rat TNF alpha antibody AAR33 bio-rad,Rabbit anti Rat TNF alpha antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rabbit anti Rat TNF alpha antibody AAR33 bio-rad,Rabbit anti Rat TNF alpha antibody

2024-10-22

Rabbit anti Rat TNF alpha antibody

货号：AAR33

规格：0.1 mg

价格：4055

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：TNF alpha

物种：大鼠

宿主：兔

抗体亚型：IgG

荧光染料：Purified

产品介绍
Rabbit anti Rat TNF alpha antibodyrecognizes rat tumor necrosis factor alpha (TNFα), also known as Cachectin or Tumor necrosis factor ligand superfamily member 2. Rat TNFα is a 235 amino acid ~17 kDa single pass type II O-linked transmembrane glycoprotein, it also exists as a secreted cytokine through cleavage between residues 79 and 80. TNFα is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells.
产品详情
Target Species	Rat
Reconstitution	Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide
Antiserum Preparation	Antisera to rat MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Product Form	Purified IgG - lyophilized
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	None present
Immunogen	Recombinant rat TNF alpha (PRP16).
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml after reconstitution.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	0.5	2.0ug/ml
Functional Assays	√	0.07	6.0ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
Description	Product Code	Applications	Pack Size
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E,WB	1mg
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C,E,WB	1mg
文献
1.Halin, S.et al.(2010) Pigment epithelium-derived factor stimulates tumor macrophage recruitment and is downregulated by the prostate tumor microenvironment.Neoplasia. 12: 336-45.2.Khandelwal, P.J.et al.(2010) Parkinson-related parkin reduces α-Synuclein phosphorylation in a gene transfer model.Mol Neurodegener. 5: 47.3.Sandoval, J.et al.(2010) Ordered transcriptional factor recruitment and epigenetic regulation of tnf-alpha in necrotizing acute pancreatitis.Cell Mol Life Sci. 67: 1687-97.4.Irwin, M.W.et al.(1999) Tissue expression and immunolocalization of tumor necrosis factor-alpha in postinfarction dysfunctional myocardium.Circulation. 99: 1492-8.5.El-Kharashi, O.A.et al.(2011) The Potential Pharmacological and Histological Benefits of Carvedilol on the Hippocampal Post- Stroke Seizures in RatsLife Science Journal, 8: 951-606.Elgarf, A.S.et al.(2014) Lipopolysaccharide repeated challenge followed by chronic mild stress protocol introduces a combined model of depression in rats: reversibility by imipramine and pentoxifylline.Pharmacol Biochem Behav. 126: 152-62.

技术参数

产品应用 elisa；wb

产品类型 Polyclonal Antibody

推荐用于 elisa；wb

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Rabbit anti Rat TNF alpha antibody AAR33 bio-rad,Rabbit anti Rat TNF alpha antibody

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