Rabbit anti Rat EGF antibody AAR37 bio-rad,Rabbit anti Rat EGF antibody

2024-10-22

Rabbit anti Rat EGF antibody

货号:AAR37

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:EGF

物种:大鼠

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Rat EGF antibodyrecognizes rat EGF (Epidermal Growth Factor), a type I transmembrane protein expressed by a variety of cells, which acts as a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, and is a key factor in cell survival.The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the EGF receptor (EGFR), which results in the triggering of several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.
产品详情
Target SpeciesRat
Product FormPurified IgG - lyophilized
Antiserum PreparationAntiserum to rat EGF was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
ReconstitutionReconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Buffer SolutionPhosphate buffered saline
Preservative StabilisersNone present
ImmunogenRecombinant rat EGF.
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml after reconstitution
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.52.0ug/ml
Functional Assays0.050.1ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg

技术参数

产品应用 elisa;wb

产品类型 Polyclonal Antibody

推荐用于 elisa;wb

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