Bio-Rad AAR15B Interleukin-1 beta,Rabbit anti Rat Interleukin-1 beta:Biotin

2024-10-22

Rabbit anti Rat Interleukin-1 beta:Biotin

货号:AAR15B

规格:50 µg

价格:4055

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Interleukin-1 beta

物种:大鼠

宿主:兔

抗体亚型:IgG

荧光染料:Biotin

产品介绍
Rabbit anti Rat Interleukin-1 beta antibodyrecognizes rat IL-1 beta. Interleukin-1β is a 152 amino acid active pro-inflammatory cytokine produced with an additional 116 amino acid pro-peptide region. IL-1&beta has a broad mode of action, stimulating prostaglandin synthesis, neutrophil, T cell and B cell activation and collagen synthesis.
产品详情

Target Species

Rat

Product Form

Purified IgG conjugated to Biotin - lyophilized

Antiserum Preparation

Antisera to rat IL-1 beta were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

None Present

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml after reconstitution

Immunogen

Recombinant rat IL-1 beta (PRP23).

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

Reconstitution

Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albuminCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

0.25

1.0ug/ml

Immunohistology - Paraffin

Western Blotting

0.10.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.ELISAThis product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together withAAR15Gas the capture reagent andPRP23as the standard.
文献

1. Girard, S. (2008) Pro-inflammatory disequilibrium of the IL-1 beta/IL-1ra ratio in an experimental model of perinatal brain damages induced by lipopolysaccharide and hypoxia-ischemia.Cytokine. 43: 54-62.2. Glatz, T.et al.(2010) Peroxisome-proliferator-activated receptors gamma and peroxisome-proliferator-activated receptors beta/delta and the regulation of interleukin 1 receptor antagonist expression by pioglitazone in ischaemic brain.J Hypertens. 28: 1488-97.3. Mahmood, J.et al.(2011) Mitigation of radiation-induced lung injury by genistein and EUK-207.Int J Radiat Biol. 87: 889-901.4. Weksler-Zangen, S.et al.(2008) Impaired glucose-stimulated insulin secretion is coupled with exocrine pancreatic lesions in the Cohen diabetic rat.Diabetes. 57: 279-87.5. Mahmood, J.et al.(2013) Mitigation of radiation-induced lung injury with EUK-207 and genistein: effects in adolescent rats.Radiat Res. 179 (2): 125-34.

技术参数

产品应用 ELISA;UHC(P);WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;UHC(P);WB

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