Bio-Rad DRAM抗体 AHP1694,Rabbit anti DRAM (C-Terminal)

2024-10-22

Rabbit anti DRAM (C-Terminal)

货号:AHP1694

规格:0.1 mg

价格:2933

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:DRAM

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍

Rabbit anti DRAM (C-terminal) antibodyrecognizes human DRAM, a lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis.DRAM is significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing.

产品详情

Target Species

Human

Species Cross-Reactivity

Target SpeciesCross Reactivity

Mouse √

Rat √

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

AntiserumPreparation

Antisera to human DRAM were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN3)

Immunogen

A peptide corresponding to a 16 amino acid sequence from near the carboxy terminus of Human DRAM.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

应用

Application Name

Verified

Min Dilution

Max Dilution

Immunohistology- Paraffin1

2.5ug/ml

Western Blotting

0.5

1.0ug/ml

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Western Blotting
  • AHP1694 detects bands of approximately 16 and 31kDa in 293 cell lysate

相关产品

Description

Product Code

Applications

Pack Size

Antigen Retrieval Buffer, pH8.0

BUF025A

P

500 ml

TidyBlot Western Blot Detection Reagent:HRP

STAR209P

WB*

0.5 ml

文献

1.Crighton D, et al. (2007) DRAM links autophagy to p53 and programmed cell death.Autophagy 3:72-42.Crighton D, et al (2006) DRAM, a p53-induced modulator of autophagy, is critical for apoptosis.Cell 126:121-34.3.Gozuacik D., et al (2004) Autophagy as a cell death and tumor suppressor mechanism. .Oncogene 23:2891-906

技术参数

产品应用 P*;WB

产品类型 Polyclonal Antibody

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://gibcohyclone.cn/thread-42884.htm
产品询价需求提交
返回
扫码添加客服微信,咨询产品报价