Bio-Rad AAM36 Interleukin-4,Rabbit anti Mouse Interleukin-4-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AAM36 Interleukin-4,Rabbit anti Mouse Interleukin-4

2024-10-22

Rabbit anti Mouse Interleukin-4

货号：AAM36

规格：0.1 mg

价格：4063

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：Interleukin-4

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：Purified

产品介绍
Rabbit anti Mouse Interleukin-4 antibodyrecognizes mouse interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, a 120 amino acid pleiotropic cytokine cleaved from a 140 amino acid precursor which interacts with multiple cell lineages, including T cells, fibroblasts and macrophages (Crawfordet al.1987).Interleukin-4 is one of the most important factors involved in B cell IgG1 and IgE class switching (Winteret al.1987). IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes (Miyajimaet al.1991,Hudaket al.1987). IL-4 acts through binding to it's cognate receptor CD124, a receptor which also binds the related cytokine IL-13.Interleukin -4 plays an important role in the alternative activation of murine macrophages as indicated by the ability to potently up-regulate the expression and activity of CD206, the macrophage mannose receptor (Steinet al.1992).
产品详情
Target Species	Mouse
Product Form	Purified IgG - lyophilized
Antiserum Preparation	Antisera to mouse IL-4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	None present
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml after reconstitution.
Immunogen	Recombinant mouse IL-4
Regulatory	For research purposes only
Guarantee	6 months from date of despatch
Reconstitution	Reconstitute with 0.1ml distilled water.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.For long term storage the addition of 0.09% sodium azide is recommended..NB: For functional studies do not add azide.
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√		0.5ug/ml
Functional Assays	√	0.25ug/ml	0.40ug/ml
Western Blotting	√	0.1ug/ml	0.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.ELISAAAM36 may be used in an indirect ELISA withPMP81as the standard.
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文献
1. de Barros, V.E.D.et al.(2011) An experimental model of meningoencephalomyelitis by Rocio flavivirus in BALB/c mice: inflammatory response, cytokine production, and histopathology.Am J Trop Med Hyg. 85 (2): 363-73.2. Kim, S.R.et al.(2013) Endoplasmic reticulum stress influences bronchial asthma pathogenesis by modulating nuclear factor κB activation.J Allergy Clin Immunol. pii: S0091-6749(13)01382-1.

技术参数

产品应用 ELISA;FN;WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;FN;WB

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Bio-Rad AAM36 Interleukin-4,Rabbit anti Mouse Interleukin-4

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