Bio-Rad AAM35 Interleukin-2,Rabbit anti Mouse Interleukin-2

2024-10-22

Rabbit anti Mouse Interleukin-2

货号:AAM35

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Interleukin-2

物种:小鼠

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Mouse Interleukin-2 antibodyrecognizes mouse Interleukin-2 (IL-2), a potent lymphoid cell growth factor which primarily acts on T lymphocytes, in response to antigenic or mitogenic stimulation.IL-2 stimulates the growth/differentiation of monocytes, macrophages, B lymphocytes, NK cells, and lymphokine activated killer cells which are used in experimental cancer therapy.
产品详情

Target Species

Mouse

Product Form

Purified IgG - lyophilized

Antiserum Preparation

Antisera to mouse IL-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

None present

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml after reconstitution

Immunogen

Recombinant mouse IL-2(PMP38)

Regulatory

For research purposes only

Guarantee

6 months from date of despatch

Reconstitution

Reconstitute with 0.1 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

0.52.0ug/ml

Functional Assays

0.040.05ug/ml

Western Blotting

0.10.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.ELISAAAM35 may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA withAAM35Bas the detection reagent andPMP38as the standard.
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文献

1. Smith, K.A. (1998) Interleukin-2: inception, impact, and implications.Science. 240: 1169-1176.

技术参数

产品应用 ELISA;FN;WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;FN;WB

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