Rabbit anti Porcine tubulin antibody 9280-0050G bio-rad,Rabbit anti Porcine tubulin antibody

2024-10-22

Rabbit anti Porcine tubulin antibody

货号:9280-0050G

规格:0.5 ml

价格:2236

产品类型:免疫组化一抗

品牌:Bio-Rad

物种:其它

宿主:兔

抗体亚型:IgG3

荧光染料:Alexa Fluor 488

产品介绍
Rabbit anti Porcine tubulin antibodyrecognizes tubulin, a protein which is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution.Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Antimicrotubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.
产品详情
Target SpeciesPig
Product FormPurified IgG - liquid
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09%Sodium Azide (NaN3)
ImmunogenPurified porcine brain tubulin.
Approx. Protein ConcentrationsIgG concentration 5.0mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining alquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA1/501/500
Western Blotting1/2501/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg
文献
1.Gelfand, V.I. & Bershadsky, A.D. (1991) Microtubule dynamics: mechanism, regulation, and function.Annu Rev Cell Biol. 7: 93-116.2.Downing, K.H. & Nogales, E. (1998) Tubulin structure: insights into microtubule properties and functions.Curr Opin Struct Biol. 8 (6): 785-91.3.Wang, M. & Collins, R.N. (2014) A lysine deacetylase Hos3 is targeted to the bud neck and involved in the spindle position checkpoint.Mol Biol Cell. 25 (18): 2720-34.4.Coluccio, L.M.et al.(2021) Enhancing Antibodies’ Binding Capacity through Oriented Functionalization of Plasmonic SurfacesNanomaterials. 11 (10): 2620.

技术参数

产品应用 elisa;wb

产品类型 Polyclonal Antibody

推荐用于 elisa;wb

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