Bio-Rad AAI22F IgG2,Sheep anti Bovine IgG2:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AAI22F IgG2,Sheep anti Bovine IgG2:FITC

2024-10-22

Sheep anti Bovine IgG2:FITC

货号：AAI22F

规格：1 mg

价格：2550

产品类型：荧光二抗

品牌：Bio-Rad

抗原：IgG2

物种：Bovine

宿主：Sheep

抗体亚型：IgG

荧光染料：FITC

产品介绍
Sheep anti Bovine IgG2 polyclonal antibodyrecognizes bovine IgG2.No cross - reactivity with other bovine immunoglobulin classes is seen in immunoelectrophoresis. This product may cross-react with IgG2 from other species.
产品详情
Target Species	Bovine
Product Form	Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation	Antisera to bovine IgG2 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 0.2% Bovine Serum Albumin
Immunogen	Purified bovine IgG2.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 monthsfrom dateof despatch
存储条件
Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/20	1/100
Immunohistology - Frozen	√	1/20	1/100
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
文献
1. Van Meulder, F.et al.(2013) Granule exocytosis of granulysin and granzyme B as a potential key mechanism in vaccine-induced immunity in cattle against the nematodeOstertagia ostertagi.Infect Immun. 81: 1798-809.2. Maree, F.F.et al.(2015) Intra-serotype SAT2 chimeric foot-and-mouth disease vaccine protects cattle against FMDV challenge.Vaccine. 33 (25): 2909-16.3. Rybarczyk, J.et al.(2017) Effects of lactoferrin treatment on Escherichia coli O157:H7 rectal colonization in cattle.Vet Microbiol. 202: 38-46.4. González-Hernández Aet al.(2016) Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response.Sci Rep. 6: 29496.5. Pecora, A.et al.(2015) Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2.Vaccine. 33 (39): 5163-71.6. Scott, K.A.et al.(2017) Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle.Vaccine. 35 (40): 5426-33.7. Sheng, Z.A.et al.(2019) Th2-related cytokines are associated withFasciola giganticainfection and evasion in the natural host, swamp buffalo.Vet Parasitol. 268: 73-80.8. Bucafusco, D .et al.(2019) Immune cells transferred by colostrum do not influence the immune responses to foot-and-mouth disease primary vaccination.J Dairy Sci. 102 (9): 8376-84.9. Jiménez-Pelayo, L.et al.(2019) EarlyNeospora caninuminfection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates.Vet Res. 50 (1): 72.10. Rainard, P.et al.(2017) Cellular and humoral immune response to recombinantEscherichia coli.OmpA in cows.PLoS One. 12 (10): e0187369.11. Springer, A.et al. (2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine LungwormDictyocaulus viviparus..Pathogens 2022, 11, 55.

技术参数

产品应用 C;F

产品类型 Polyclonal Antibody

推荐用于 C;F

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Bio-Rad AAI22F IgG2,Sheep anti Bovine IgG2:FITC

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