Bio-Rad AAI21AB IgG1,Sheep anti Bovine IgG1:Alk. Phos.

2024-10-22

Sheep anti Bovine IgG1:Alk. Phos.

货号:AAI21AB

规格:0.5 mg

价格:2635

产品类型:荧光二抗

品牌:Bio-Rad

抗原:IgG1

物种:Bovine

宿主:Sheep

抗体亚型:IgG

荧光染料:Alkaline Phosphatase

产品介绍
Sheep anti Bovine IgG1 polyclonal antibodyrecognizes bovine IgG1.No cross-reactivity with other bovine immunoglobulin classes is seen in immunoelectrophoresis.This product may cross-react with IgG1 from other species.
产品详情

Target Species

Bovine

Product Form

Purified IgG conjugated to Alkaline Phosphatase - liquid

Antiserum PreparationAntisera to bovine IgM were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.

BufferSolution

50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

Immunogen

Purified bovine IgG1.

Approx. Protein ConcentrationsIgG concentration 0.5mg/ml

Regulatory

For research purposes only

Guarantee

12 monthsfrom dateof despatch

存储条件
Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution

ELISA

1/10001/10000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
文献
1. Maree, F.F.et al.(2015) Intra-serotype SAT2 chimeric foot-and-mouth disease vaccine protects cattle against FMDV challenge.Vaccine. 33 (25): 2909-16.2. Rybarczyk, J.et al.(2017) Effects of lactoferrin treatment on Escherichia coli O157:H7 rectal colonization in cattle.Vet Microbiol. 202: 38-46.3. Bautista-Garfias, C.R.et al.(2015) Co-immunization of cattle with a vaccine against babesiosis andLactobacillus caseiincreases specific IgG1 levels toBabesia bovisandB. bigemina.Parasitol Int. 64 (5): 319-23.4. González-Hernández Aet al.(2016) Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response.Sci Rep. 6: 29496.5. Scott, K.A.et al.(2017) Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle.Vaccine. 35 (40): 5426-33.6. Sheng, Z.A.et al.(2019) Th2-related cytokines are associated withFasciola giganticainfection and evasion in the natural host, swamp buffalo.Vet Parasitol. 268: 73-80.7. Jiménez-Pelayo, L.et al.(2019) EarlyNeospora caninuminfection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates.Vet Res. 50 (1): 72.8. Rainard, P.et al.(2017) Cellular and humoral immune response to recombinantEscherichia coli.OmpA in cows.PLoS One. 12 (10): e0187369.9. Hine, B.C.et al.(2019) Immune competence traits assessed during the stress of weaning are heritable and favorably genetically correlated with temperament traits in Angus cattle.J Anim Sci. 97 (10): 4053-4065.10. Springer, A.et al. (2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine LungwormDictyocaulus viviparus..Pathogens 2022, 11, 55.

技术参数

产品应用 ELISA

产品类型 Polyclonal Antibody

推荐用于 ELISA

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