Mouse anti Human CD140b antibody, clone PR7212 7460-3104 ,Mouse anti Human CD140b antibody, clone PR7212

2024-10-22

Mouse anti Human CD140b antibody, clone PR7212

货号:7460-3104

规格:0.1 mg

价格:3341

产品类型:免疫组化一抗

品牌:Bio-Rad

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:PR7212

荧光染料:Purified

产品介绍
Mouse anti Human CD140b antibody, clone PR7212recognizes the extracellular domain of the human PDGF receptor beta subunit. This was confirmed in binding studies using several different cell lines and by its ability to immunoprecipitate PDGF receptor beta complexed with125I-PDGF-BB (Hartet al.1987). Mouse anti Human CD140b antibody, clone PR7212 does not recognize PDGF receptor alpha.PDGF receptor beta is a member of the class III receptor tyrosine kinase family that also includes M-CSF receptor, SCF receptor and Flt-3. Binding of PDGF-BB induces receptor homodimerization or heterodimerization with PDGF receptor alpha.
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from ascites
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers<0.1% Sodium Azide (NaN3)
ImmunogenHuman skin fibroblast cell membrane extracts.
Approx. Protein Concentrations1.0 mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry25 μg/ml
Immunofluorescence
Western Blotting
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117FF0.5mg
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117AE,WB0.5mg
文献
1.Hart, C.E.et al.(1987) Synthesis, phosphorylation, and degradation of multiple forms of the platelet-derived growth factor receptor studied using a monoclonal antibody.J Biol Chem. 262: 10780-5.2.Hart, C.E.et al.(1988) Two classes of PDGF receptor recognize different isoforms of PDGF.Science. 240: 1529-31.3.Franklin, W.A.et al.(1990) In situ distribution of the beta-subunit of platelet-derived growth factor receptor in nonneoplastic tissue and in soft tissue tumors.Cancer Res. 50: 6344-8.4.Seifert, R.A.et al.(1989) Two different subunits associate to create isoform-specific platelet-derived growth factor receptors.J Biol Chem. 264: 8771-8.5.Krane, J.F.et al.(1991) Increased dermal expression of platelet-derived growth factor receptors in growth-activated skin wounds and psoriasis.J Invest Dermatol. 96: 983-6.

技术参数

产品应用 wb

产品类型 Monoclonal Antibody

推荐用于 wb

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