Bio-Rad TRIM5 Alpha抗体 AHP1582,Goat anti Human TRIM5 Alpha (N-Terminal)

2024-10-22

Goat anti Human TRIM5 Alpha (N-Terminal)

货号:AHP1582

规格:0.1 mg

价格:5083

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:TRIM5 Alpha

物种:人

宿主:山羊

抗体亚型:IgG

荧光染料:Purified

产品介绍

Goat anti Human TRIM5 alpha antibodyrecognizes an epitope within the N-terminal (NT) region of human TRIM5alpha, otherwise known as RNF88 (RING finger protein 88), a cytoplasmic protein and member of the TRIM/RBCC family, known to play a vital role in species-specific early defence against retrovirus infection.The association of TRIM5 with specific retroviral capsid sequences determines the effectiveness of the defence against infection. For example, in Old World monkeys, TRIM5alpha specifically associates with HIV type 1 (HIV-1), whereas in humans TRIM5alpha is not effective against HIV-1, but can inhibit strains of the murine leukaemia virus (MLV).

产品详情

Target Species

Human

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to human TRIM5alpha (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin

Immunogen

Peptide sequence ASGILVNVKEEVTC corresponding to the N-Terminal region of TRIM5 (NP_149023.1; NP_149083.1; NP_149084.1).

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

1/16000

Immunohistology - Paraffin1

1.0ug/ml

Western Blotting

1.0

3.0ug/ml

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Histology Positive Control Tissue
  • Human tonsil
  • Western Blotting
  • AHP1582 detects a band of approximately 37kDa in human brain cell lysates.

可用二抗

Description

Product Code

Applications

Pack Size

Rabbit anti Goat IgG (Fc):FITC

STAR122F

F

1 mg

Rabbit anti Goat IgG (Fc):HRP

STAR122P

C E WB

1 mg

相关产品

Description

Product Code

Applications

Pack Size

Antigen Retrieval Buffer, pH8.0

BUF025A

P

500 ml

文献

1.Lee, K. & Kewalramani, V.N. (2004) In defense of the cell: TRIM5alpha interception of mammalian retroviruses.Proc Natl Acad Sci U S A. 101 (29): 10496-7.

技术参数

产品应用 E ;P* ;WB

产品类型 Polyclonal Antibody

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