Bio-Rad 4670-5107 Glutamate Receptor 1 (C-Terminal),Rabbit anti Rat Glutamate Receptor 1 (C-Terminal)

2024-10-22

Rabbit anti Rat Glutamate Receptor 1 (C-Terminal)

货号:4670-5107

规格:50 µg

价格:6010

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Glutamate Receptor 1 (C-Terminal)

物种:大鼠

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Rat Glutamate Receptor 1 (C-Terminal) antibodyrecognizes the glutamate receptor 1 (also known as AMPA) which acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.This antibody linked to immobilized Protein A immunoprecipitates detergent-solubilized receptor from brain or transfected cells.
产品详情

Target Species

Rat

Product Form

Purified Ig - liquid

Preparation

Purified Ig prepared by affinity chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

<0.1% Sodium Azide (NaN3)

Approx. Protein Concentrations

Ig concentration 1 mg/ml

Immunogen

Synthetic peptide corresponding to the C terminal region (SHSSGMPLGATGL) of the native molecule conjugated to Bovine Serum Albumin.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用

Application Name

Verified

Min Dilution

Max Dilution

Immunohistology - Frozen

1 µg/ml

3 µg/ml

Immunoprecipitation

Western Blotting

0.5 µg/ml2.5 µg/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.ImmunohistologyParaformaldehyde or paraformaldehyde/glutaraldehyde are suitable. Cryostat and vibratome sections can be used with or without Triton X-100 treatment.Western BlottingWestern blot analysis of rat brain shows a single band of approximately 110 kDa co-migrating with GluR1 expressed in transfected cells.
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文献

1. Petralia, R.S. & Wenthold, R.J. (1992) Light and electron immunocytochemical localization of AMPA-selective glutamate receptors in the rat brain.J Comp Neurol. 318 (3): 329-54.2. Wenthold, R.J.et al.(1992) Immunochemical characterization of the non-NMDA glutamate receptor using subunit-specific antibodies. Evidence for a hetero-oligomeric structure in rat brain.J Biol Chem. 267 (1): 501-7.

技术参数

产品应用 IHC(F);IP;WB

产品类型 Polyclonal Antibody

推荐用于 IHC(F);IP;WB

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