Bio-Rad 4600-1704,Native Escherichia coli Beta Galactosidase

2024-10-22

Native Escherichia coli Beta Galactosidase

货号:4600-1704

规格:5 mg

价格:3460

产品类型:蛋白

品牌:Bio-Rad

产品介绍
Escherichia coliBeta-Galactosidase is an inducible tetrameric enzyme coded by the lac Z gene of the lac operon that is often used as a reporter to assess the efficiency of transfection. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
产品详情

Target Species

Bacterial

Product Form

Purified protein fromEscherichia coli- lyophilized

Reconstitution

Reconstitute with 1.0 ml sterile distilled water or aqueous buffer of choice.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

Contains Sucrose

Approx. Protein Concentrations

Protein content is approximately 20-25% of total weight

Activity

>600 U/mg proteinOne unit hydrolyses 1 µmole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37ºC.For batch-specific activity, please inquire.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

Protein Molecular Weight

540 kDa
存储条件
Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

Functional Assays

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 FN

产品类型 Purified Protein

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