Bio-Rad 4510-7864 FITC,Rabbit anti FITC:HRP

2024-10-22

Rabbit anti FITC:HRP

货号:4510-7864

规格:1 ml

价格:5398

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原: FITC

物种:Chemical

宿主:兔

抗体亚型:IgG

荧光染料:HRP

产品介绍
Rabbit anti FITC antibodyrecognizes fluorescein and does not react with immunoglobulins from any species.Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins.
产品详情

Target Species

Chemical

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Preparation

Purified IgG prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Thiomersal1% Bovine serum albumin.

Approx. Protein Concentrations

1.0 mg/ml

Immunogen

FITC Isomer 1.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

1/200

1/1000

Western Blotting

1/201/200

Immunohistology - Frozen

1/201/200
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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数据

Published customer image:Rabbit anti FITC antibody (4510-7804) used as a visualization antibody in an ELISA assay for the detection of antiFasciola hepaticaantibodies in human sera.Image caption:Comparison of the detection limit of the MM3-SERO and SeroFluke tests. The ability of MM3-SERO ELISA (A) and SeroFluke (B) to detect anti-Fasciola antibodies in two fold dilutions of pooled positive sera was compared. The starting serum dilution was 1/100 for both tests.
文献

1. van der Loos, C. (2008) Multiple immunoenzyme staining: methods and visualizations for the observation with spectral imaging.J. Histochem Cytochem. 56: 313-328.2. Bdeir, K.et al.(2010) Neutrophil alpha-defensins cause lung injury by disrupting the capillary-epithelial barrier.Am J Respir Crit Care Med.181: 935-46.3. Hsiao, J.R.et al.(2010) Cyclic alphavbeta6-targeting peptide selected from biopanning with clinical potential for head and neck squamous cell carcinoma.Head Neck. 32(2): 160-72.4. Türeci Oet al.(2011) Claudin-18 gene structure, regulation, and expression is evolutionary conserved in mammals.Gene. 481 (2): 83-92.5. Martínez-Sernández, V.et al.(2011) Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis.PLoS Negl Trop Dis. 5 (11):e1376.

技术参数

产品应用 IHC(F);ELISA;WB

产品类型 Polyclonal Antibody

推荐用于 IHC(F);ELISA;WB

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