Mouse anti Cryptosporidium antibody, clone BEL 0126 bio-rad,Mouse anti Cryptosporidium antibody, clone BEL 0126

2024-10-22

Mouse anti Cryptosporidium antibody, clone BEL 0126

货号:2402-3007A488

规格:100 Tests

价格:4038

产品类型:流式抗体

品牌:Bio-Rad

物种:Protozoan

宿主:小鼠

抗体亚型:IgG3

克隆号:BEL 0126

荧光染料:Alexa Fluor 488

产品介绍
Mouse anti Cryptosporidium antibody, clone BEL 0126recognizes a membrane antigen expressed by the oocysts ofCryptosporidiumsp., an obligate enteric coccidian parasite of the phylum Apicomplexa that infects the gastrointestinal tract. The parasite is one of the most important enteric pathogens in both humans and animals (Roseet al.2002). Since its first diagnosis in 1975 (Meiselet al.1976), Cryptosporidiosis, has become one of the most prominent public health concerns worldwide (Roseet al.2002).Cryptosporidiumoocysts are resistant to chlorine and their small size makes removal by filtration difficult. Alternative methods have been developed such as UV and ozone treatment alongside monitoring using Immunofluorescence screening (Roseet al.2002).Cryptosporidiosis is a disease affecting the intestines of mammals which is spread through the fecal-oral route. The main symptom of is self-limiting diarrhea in people with intact immune systems. However, in immunocompromised individuals, such as AIDS patients, infection can cause permanent & life-threatening diarrhea (Maet al.1984).
产品详情
Target SpeciesProtozoan
Product FormPurified IgG conjugated to Alexa Fluor®488 - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09%Sodium Azide (NaN3)1%Bovine serum albumin
ImmunogenPurified cryptosporidium oocysts from bovine faeces.
Approx. Protein ConcentrationsIgG concentration 0.05mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®488495519
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/51/20
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Am J Pathol. 150: 1395-405.2.Weigum, S.E.et al.(2016) Hollow silica microspheres for buoyancy-assisted separation of infectious pathogens from stool.J Chromatogr A. 1466: 29-36.3.Luka, G.et al.(2019) Label-Free Capacitive Biosensor for Detection ofCryptosporidium.Sensors (Basel). 19 (2)Jan 10 [Epub ahead of print].4.Sonzogni-desautels, K.et al.(2019) A protocol to countCryptosporidiumoocysts by flow cytometry without antibody staining.PLoS Negl Trop Dis. 13 (3): e0007259.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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